An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins.
Leptospira
adherence
cadherin
infection
leucine-rich
spirochaete
Journal
Cellular microbiology
ISSN: 1462-5822
Titre abrégé: Cell Microbiol
Pays: India
ID NLM: 100883691
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
17
05
2018
revised:
20
08
2018
accepted:
24
08
2018
pubmed:
2
9
2018
medline:
19
3
2020
entrez:
2
9
2018
Statut:
ppublish
Résumé
Pathogenic Leptospira bacteria are the causative agents of leptospirosis, a zoonotic disease affecting animals and humans worldwide. These pathogenic species have the ability to rapidly cross host tissue barriers by a yet unknown mechanism. A comparative analysis of pathogens and saprophytes revealed a higher abundance of genes encoding proteins with leucine-rich repeat (LRR) domains in the genomes of pathogens. In other bacterial pathogens, proteins with LRR domains have been shown to be involved in mediating host cell attachment and invasion. One protein from the pathogenic species Leptospira interrogans, LIC10831, has been previously analysed via X-ray crystallography, with findings suggesting it may be an important bacterial adhesin. Herein we show that LIC10831 elicits an antibody response in infected animals, is actively secreted by the bacterium, and binds human E- and VE-cadherins. These results provide biochemical and cellular evidences of LRR protein-mediated host-pathogen interactions and identify a new multireceptor binding protein from this infectious Leptospira species.
Identifiants
pubmed: 30171791
doi: 10.1111/cmi.12949
pmc: PMC7560960
mid: NIHMS1505424
doi:
Substances chimiques
Adhesins, Bacterial
0
Antigens, CD
0
CDH1 protein, human
0
Cadherins
0
Leucine-Rich Repeat Proteins
0
Proteins
0
cadherin 5
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12949Subventions
Organisme : NIAID NIH HHS
ID : R01 AI112920
Pays : United States
Informations de copyright
© 2018 John Wiley & Sons Ltd.
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