Synthesis of mycotoxin protein IF8 by the entomopathogenic fungus Isaria fumosorosea and its toxic effect against adult Diaphorina citri.

Animals Ascomycota / metabolism Chemical Phenomena Fermentation Hemiptera / drug effects Insecticides / chemistry Liquid-Liquid Extraction Metabolome Metabolomics / methods Models, Molecular Molecular Weight Mycotoxins / biosynthesis Protein Conformation
Insect control Isaria fumosorosea Liquid fermentation Physico-chemical properties Toxic protein

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 Mar 2019
Historique:
received: 04 04 2018
revised: 12 09 2018
accepted: 15 09 2018
pubmed: 19 9 2018
medline: 21 5 2019
entrez: 19 9 2018
Statut: ppublish

Résumé

Entomopathogenic fungi based microbial insecticides are considered as safe alternatives to chemical pesticides, which secretes several bioactive compounds to kill the host insects. In this study, we report a new approach for the synthesis and characterization of insecticide toxic protein IF8 produced by the Isaria fumosorosea 08, and to evaluate the mycotoxin level against the vector of Huanglongbing (HLB) or citrus greening disease, the Asian citrus psyllid, Diaphorina citri. Soluble toxic metabolites extracted from I. fumosorosea 08 through submerged liquid state culture had a molecular weight of 43 kDa when subjected by to sodium dodecyl sulfate-poly-acrylamide (SDS-PAGE) gel electrophoresis. The most abundant of toxic protein IF8 was determined by High-performance liquid chromatography (HPLC) and liquid chromatography electrospray ionization-mass spectroscopy (LC-ESI-MS) for the analysis of its molecular mass weight and purity. Further Matrix-assisted laser desorption ionization-time of flight (MALDI-TOFF) analysis confirmed the presence of toxic metabolites in liquid culture. Subsequently, mycotoxic effect of toxic protein IF8 was tested against D. citri at three different concentrations (1%, 2%, and 3%). The results showed the insecticidal activity of >80% when administered at three different concentrations at 48-120 hour post-application. Additionally, we also investigated the physicochemical properties and stability of IF8 by using computational biological tools. This is the first study to report the characterization of fungal mediated synthesis of the protein IF8 toxic to the insect D. citri. These results suggest the mycotoxin control of D. citri and prevention of HLB transmission by using a natural toxic compound which is eco-friendly and can be potentially used for the integrated management of D. citri.

Identifiants

DOI: 10.1016/j.ijbiomac.2018.09.093 PMID: 30227211
pubmed: 30227211
pii: S0141-8130(18)31578-2
doi: 10.1016/j.ijbiomac.2018.09.093
pii:
doi:

Substances chimiques

Insecticides 0
Mycotoxins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1203-1211

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Ravindran Keppanan (R)

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China; College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China.

Patcharin Krutmuang (P)

Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand.

Sivaramakrishnan Sivaperumal (S)

Department of Biotechnology and Genetic Engineering, School of Life Sciences, Bharathidasan University, Tamil Nadu, India.

Mubasher Hussain (M)

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China; College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China; College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, China.

Bamisope Steve Bamisile (BS)

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China; College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China.

Luis Carlos Ramos Aguila (LCR)

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China; College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China.

Chandra Kanta Dash (CK)

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China; College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China.

Liande Wang (L)

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China; College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China; Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, China. Electronic address: Liande_wang@126.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Eucaryotes › Champignons › Ascomycota › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Phénomènes chimiques › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Hemiptera › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Pesticides › Insecticides › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Fractionnement chimique › Extraction liquide-liquide › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Métabolisme › Métabolome › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Métabolomique › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Phénomènes chimiques › Masse moléculaire › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Mycotoxines › Synthesis of mycotoxin protein IF8 by the...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Synthesis of mycotoxin protein IF8 by the...