The dual sex hormone specificity for human reductive 17β-hydroxysteroid dehydrogenase type 7: Synergistic function in estrogen and androgen control.
17β-hydroxysteroid dehydrogenase
Breast cancer
Enzymology
Journal
The Journal of steroid biochemistry and molecular biology
ISSN: 1879-1220
Titre abrégé: J Steroid Biochem Mol Biol
Pays: England
ID NLM: 9015483
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
16
03
2018
revised:
07
09
2018
accepted:
12
09
2018
pubmed:
19
9
2018
medline:
22
5
2019
entrez:
19
9
2018
Statut:
ppublish
Résumé
Human 17β-hydroxysteroid dehydrogenase (17β-HSD) type 1 and 7 catalyze the final step of estrogen activation and the first step in androgen inactivation. It has been shown in breast cancer cells that DHT has a suppression effect on cell proliferation, counteracting the estrogen growth effect. However, the exact kinetic function of 17β-HSD7 in steroidogenesis was not determined. Here we report the steady-state kinetics and binding study for 17β-HSD7 with estrone or DHT as substrates and NADPH as cofactor. 17β-HSD7 has been overexpressed in E. coli and purified. For both substrates, kinetics of 17β-HSD7 demonstrates positive cooperativity. The K
Identifiants
pubmed: 30227243
pii: S0960-0760(18)30165-1
doi: 10.1016/j.jsbmb.2018.09.012
pii:
doi:
Substances chimiques
Androgens
0
Estrogens
0
Dihydrotestosterone
08J2K08A3Y
Estrone
2DI9HA706A
NADP
53-59-8
17-Hydroxysteroid Dehydrogenases
EC 1.1.-
3 (or 17)-beta-hydroxysteroid dehydrogenase
EC 1.1.1.51
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
61-65Subventions
Organisme : CIHR
ID : MOP 97917
Pays : Canada
Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.