Generation of a potential koi herpesvirus live vaccine by simultaneous deletion of the viral thymidine kinase and dUTPase genes.
Animals
Carps
/ immunology
Cells, Cultured
DNA, Viral
/ genetics
Fish Diseases
/ immunology
Herpesviridae
/ genetics
Herpesviridae Infections
/ immunology
Israel
Pyrophosphatases
/ genetics
Sequence Deletion
/ genetics
Thymidine Kinase
/ genetics
Vaccines, Attenuated
/ genetics
Virus Replication
/ genetics
Cyprinid herpesvirus 3
dUTPase
koi herpesvirus
thymidine kinase
vaccine
Journal
The Journal of general virology
ISSN: 1465-2099
Titre abrégé: J Gen Virol
Pays: England
ID NLM: 0077340
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
pubmed:
20
9
2018
medline:
19
12
2019
entrez:
20
9
2018
Statut:
ppublish
Résumé
Koi herpesvirus (KHV, Cyprinidherpesvirus 3) causes a fatal disease of koi and common carp. To obtain safe and efficacious live vaccines, we generated deletion mutants of KHV lacking the nonessential genes encoding two enzymes of nucleotide metabolism, thymidine kinase (TK, ORF55) and deoxyuridine-triphosphatase (DUT, ORF123). Since single-deletion mutants based on a KHV isolate from Israel (KHV-I) only exhibited partial attenuation (Fuchs W, Fichtner D, Bergmann SM, Mettenleiter TC. Arch Virol 2011;156 : 1059-1063), a corresponding double mutant was generated and tested in vivo, and shown to be almost avirulent but still protective. To overcome the low in vitro virus titres of KHV-I (≤10
Identifiants
pubmed: 30230443
doi: 10.1099/jgv.0.001148
doi:
Substances chimiques
DNA, Viral
0
Vaccines, Attenuated
0
Thymidine Kinase
EC 2.7.1.21
Pyrophosphatases
EC 3.6.1.-
dUTP pyrophosphatase
EC 3.6.1.23
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM