Optimising the transient expression of GABA(A) receptors in adherent HEK293 cells.
GABA(A) receptor
HEK293
Ligand-gated ion channel
Transient expression
Journal
Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
22
06
2018
revised:
17
09
2018
accepted:
20
09
2018
pubmed:
25
9
2018
medline:
24
1
2020
entrez:
25
9
2018
Statut:
ppublish
Résumé
Owing to their therapeutic relevance, considerable efforts are devoted to the structural characterisation of membrane proteins. Such studies are limited by the availability of high quality protein due to the difficulty of overexpression in recombinant mammalian systems. We sought to systematically optimise multiple aspects in the process of transiently transfecting HEK293 cells, to allow the rapid expression of membrane proteins, without the lengthy process of stable clone formation. We assessed the impact of medium formulation, cell line, and harvest time on the expression of GABA
Identifiants
pubmed: 30248449
pii: S1046-5928(18)30350-4
doi: 10.1016/j.pep.2018.09.012
pii:
doi:
Substances chimiques
Receptors, GABA-A
0
Recombinant Proteins
0
Muscimol
2763-96-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7-15Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.