Phenotypic and genotypic analyses of antimicrobial resistant bacteria in livestock in Uganda.
Escherichia coli
Antimicrobial resistant bacteria
Uganda
livestock
metagenomic analysis
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
22
02
2018
revised:
03
09
2018
accepted:
13
09
2018
pubmed:
28
9
2018
medline:
16
3
2019
entrez:
28
9
2018
Statut:
ppublish
Résumé
Antimicrobial resistant bacteria (ARB) in livestock are a global public health concern, not only because they prolong infectious diseases but also they can be transferred from animals to humans via the food chain. Here, we studied ARB in livestock at commercial and subsistence farms (n = 13) in Wakiso and Mpigi districts, Uganda. We enquired from the farmers about the type and the purpose of antimicrobial agents they have used to treat their livestock. After collecting faeces, we isolated antimicrobial resistant Escherichia coli from livestock faeces (n = 134) as an indicator bacterium. These strains showed resistance to ampicillin (44.8%), tetracycline (97.0%), and sulfamethoxazole-trimethoprim (56.7%). The frequency of ampicillin-resistance was significantly correlated with the usage of penicillins to livestock in the farms (p = 0.04). The metagenomics data detected 911 antimicrobial resistant genes that were classified into 16 categories. Genes for multidrug efflux pumps were the most prevalent category in all except in one sample. Interestingly, the genes encoding third-generation cephalosporins (bla
Substances chimiques
Anti-Infective Agents
0
Banques de données
GENBANK
['AIT76104.1', 'AAG44570.1', 'BAC76072.1', 'AAC45086.2', 'AFU25650.1', 'AHL39333.1', 'ABK33456.1', 'AAC23556.1', 'AIJ27543.1', 'NP_415632.1', 'BAA16547.1', 'NP_417544.5', 'CAA37805.1', 'AAL08441.1', 'CAA23899.1', 'ACC85616.1', 'AJE60855.1', 'CCE36834', 'ABF66027.1', 'AAY32951.1', 'NP_899166.1', 'YP008997285.1', 'AAC45607.1', 'AAC36915.1', 'ACA23185.1', 'AAO16462.1', 'AFK31666.1', 'AAC75315.1', 'BAE78116.1', 'YP_492255.1', 'ABA71727.1', 'ABA71729.1', 'WP_011461303', 'CBY88983.1', 'AAA86871.1', 'AAF86220.1', 'AAL59753.1', 'AEJ33969.1', 'WP_063855569', 'ACI32877.1', 'YP_001715373.1', 'AAD01868.1', 'AAC75136.1', 'AAC75137.1', 'AAC76539.1']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
317-326Subventions
Organisme : Japan Society for the Promotion of Science
ID : 16H01782
Organisme : Japan Society for the Promotion of Science
ID : 16H02767
Organisme : DELTAS Africa Initiative grant
ID : # DEL-15-011
Informations de copyright
© 2018 Blackwell Verlag GmbH.