Development and validation of an RT-qPCR assay for rapid detection and quantification of hepatitis C virus RNA for routine testing in Moroccan clinical specimens.
5' Untranslated Regions
Clinical Laboratory Techniques
/ methods
DNA Primers
/ genetics
Genotype
Hepacivirus
/ genetics
Hepatitis C
/ blood
Humans
Limit of Detection
Molecular Diagnostic Techniques
/ methods
Morocco
RNA, Viral
/ genetics
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
/ methods
Sensitivity and Specificity
Viral Load
/ methods
Hepatitis C
RT-qPCR
molecular diagnostic
viral load quantification
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
09
08
2018
accepted:
24
09
2018
pubmed:
30
9
2018
medline:
11
2
2020
entrez:
30
9
2018
Statut:
ppublish
Résumé
A one-step reverse transcription quantitative PCR (RT-qPCR) assay in combination with rapid RNA extraction was evaluated for routine testing of hepatitis C virus (HCV) RNA. Specific primers and probes were designed for the detection of a 150 bp sequence located in the 5'untranslated region (5'UTR) of HCV RNA. The target sequence was selected as the most conserved region between the six known HCV subtype sequences following an alignment. The assay was able to quantify a dynamic linear range of 10
Substances chimiques
5' Untranslated Regions
0
DNA Primers
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
428-436Subventions
Organisme : Moroccan foundation for advanced science, innovation and research (MAScIR)
Pays : International
Informations de copyright
© 2018 Wiley Periodicals, Inc.