Protein-Specific Analysis of Invertebrate Glycoproteins.
Animals
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Epitope Mapping
Epitopes
/ chemistry
Glycoproteins
/ metabolism
Invertebrates
Peptides
/ metabolism
Polysaccharides
/ chemistry
Proteolysis
Proteome
Proteomics
/ methods
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Glycoproteomics
Glycosylation
Mass spectrometry
“off-line” MALDI-TOF MS(MS)
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
3
10
2018
pubmed:
3
10
2018
medline:
5
6
2019
Statut:
ppublish
Résumé
N-Glycans are posttranslational modifications of proteins attached to the amide side chains of asparagine residues, with possible heterogeneity due to different structures being possible at the same glycosylation site. In contrast to the mammalian systems, invertebrate N-glycosylation presents a challenge in analysis as there exist unfamiliar epitopes and a high degree of structural and isomeric variation between different species. A simple analytical approach to analyze N-glycans on specific glycoproteins is presented, which involves a combination of tryptic peptide mass spectrometry and "off-line" RP-HPLC MALDI-TOF MS/MS complemented by blotting to recognize specific epitopes. An additional N-glycan enrichment and labeling step can facilitate the analysis of single structures and even provide isomeric separation of N-glycans from specific proteins.
Identifiants
pubmed: 30276752
doi: 10.1007/978-1-4939-8814-3_24
pmc: PMC6345373
mid: EMS81412
doi:
Substances chimiques
Epitopes
0
Glycoproteins
0
Peptides
0
Polysaccharides
0
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
421-435Subventions
Organisme : Austrian Science Fund FWF
ID : P 23922
Pays : Austria
Organisme : Austrian Science Fund FWF
ID : P 25058
Pays : Austria
Organisme : Austrian Science Fund FWF
ID : P 26662
Pays : Austria
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