L-carnitine prevents ammonia-induced cytotoxicity and disturbances in intracellular amino acid levels in human astrocytes.
Amino Acids, Branched-Chain
/ metabolism
Ammonium Chloride
/ toxicity
Astrocytes
/ drug effects
Carnitine
/ pharmacology
Cell Line
Cell Survival
/ drug effects
Cytoprotection
Glutamine
/ metabolism
Humans
L-Lactate Dehydrogenase
/ metabolism
Metabolomics
/ methods
Neuroprotective Agents
/ pharmacology
Oxidative Stress
/ drug effects
Reactive Oxygen Species
/ metabolism
branched-chain amino acids
carnitine
human astrocyte
hyperammonemia
oxidative stress
Journal
Journal of gastroenterology and hepatology
ISSN: 1440-1746
Titre abrégé: J Gastroenterol Hepatol
Pays: Australia
ID NLM: 8607909
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
06
03
2018
revised:
04
09
2018
accepted:
19
09
2018
pubmed:
3
10
2018
medline:
30
1
2020
entrez:
3
10
2018
Statut:
ppublish
Résumé
L-carnitine (L-CA) has been used therapeutically to treat hepatic encephalopathy with hyperammonemia, but the mechanism by which L-CA contributes to ammonia detoxification in the brain is still unclear. Thus, the cytotoxicity and changes in intracellular amino acids (AAs) in astrocytes with hyperammonemia following L-CA administration were studied. Human astrocytes were treated with ammonium chloride (NH Intracellular total reactive oxygen species and lactate dehydrogenase leakage were significantly increased after treatment with NH L-CA protects human astrocytes from ammonia-induced acute cytotoxic effects and the increased intracellular levels of glutamine and BCAAs.
Sections du résumé
BACKGROUND AND AIM
OBJECTIVE
L-carnitine (L-CA) has been used therapeutically to treat hepatic encephalopathy with hyperammonemia, but the mechanism by which L-CA contributes to ammonia detoxification in the brain is still unclear. Thus, the cytotoxicity and changes in intracellular amino acids (AAs) in astrocytes with hyperammonemia following L-CA administration were studied.
METHODS
METHODS
Human astrocytes were treated with ammonium chloride (NH
RESULTS
RESULTS
Intracellular total reactive oxygen species and lactate dehydrogenase leakage were significantly increased after treatment with NH
CONCLUSION
CONCLUSIONS
L-CA protects human astrocytes from ammonia-induced acute cytotoxic effects and the increased intracellular levels of glutamine and BCAAs.
Substances chimiques
Amino Acids, Branched-Chain
0
Neuroprotective Agents
0
Reactive Oxygen Species
0
Ammonium Chloride
01Q9PC255D
Glutamine
0RH81L854J
L-Lactate Dehydrogenase
EC 1.1.1.27
Carnitine
S7UI8SM58A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1249-1255Subventions
Organisme : Grant-in-Aid for Scientific Research in Japan
ID : 25461009
Informations de copyright
© 2018 Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd.