miR-191 modulates B-cell development and targets transcription factors E2A, Foxp1, and Egr1.
Animals
B-Lymphocytes
/ physiology
Basic Helix-Loop-Helix Transcription Factors
/ genetics
Cell Differentiation
Cells, Cultured
Early Growth Response Protein 1
/ genetics
Forkhead Transcription Factors
/ genetics
Gene Regulatory Networks
Mice
Mice, Inbred C57BL
Mice, Knockout
MicroRNAs
/ genetics
RNA, Small Interfering
/ genetics
Recombination, Genetic
Repressor Proteins
/ genetics
Transcription, Genetic
Transgenes
/ genetics
B cells
Lymphocyte development
Transcriptional factors
miR-191
miRNA
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
24
04
2018
revised:
17
09
2018
accepted:
01
10
2018
pubmed:
4
10
2018
medline:
29
5
2019
entrez:
4
10
2018
Statut:
ppublish
Résumé
The interdependence of posttranscriptional gene regulation via miRNA and transcriptional regulatory networks in lymphocyte development is poorly understood. Here, we identified miR-191 as direct upstream modulator of a transcriptional module comprising the transcription factors Foxp1, E2A, and Egr1. Deletion as well as ectopic expression of miR-191 resulted in developmental arrest in B lineage cells, indicating that fine tuning of the combined expression levels of Foxp1, E2A, and Egr1, which in turn control somatic recombination and cytokine-driven expansion, constitutes a prerequisite for efficient B-cell development. In conclusion, we propose that miR-191 acts as a rheostat in B-cell development by fine tuning a key transcriptional program.
Identifiants
pubmed: 30281154
doi: 10.1002/eji.201847660
doi:
Substances chimiques
Basic Helix-Loop-Helix Transcription Factors
0
Early Growth Response Protein 1
0
Egr1 protein, mouse
0
Forkhead Transcription Factors
0
Foxp1 protein, mouse
0
MIRN191 microRNA, mouse
0
MicroRNAs
0
RNA, Small Interfering
0
Repressor Proteins
0
Tcf3 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
121-132Informations de copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.