Highly Efficient Synthesis and Assay of Protein-Imprinted Nanogels by Using Magnetic Templates.
competitive assays
molecularly imprinted polymers
nanogel synthesis
plastic antibodies
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
14 01 2019
14 01 2019
Historique:
received:
22
05
2018
revised:
10
09
2018
pubmed:
12
10
2018
medline:
29
7
2020
entrez:
12
10
2018
Statut:
ppublish
Résumé
We report an approach integrating the synthesis of protein-imprinted nanogels ("plastic antibodies") with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino-functionalized solgel-coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high-dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised by a novel competitive fluorescence assay employing identical protein-conjugated nanoparticles as ligands to reversibly immobilize the corresponding nanogels. Both nanogels exhibited K
Identifiants
pubmed: 30308085
doi: 10.1002/anie.201805772
pmc: PMC6471088
doi:
Substances chimiques
Acrylic Resins
0
Enzymes, Immobilized
0
Magnetite Nanoparticles
0
Nanogels
0
polyacrylamide
9003-05-8
Trypsin
EC 3.4.21.4
Pepsin A
EC 3.4.23.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
727-730Subventions
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : H2020-MSCA-ITN-2016, 722171
Pays : International
Organisme : Stiftelsen för Kunskaps- och Kompetensutveckling
ID : 20150086
Pays : International
Organisme : Stiftelsen för Kunskaps- och Kompetensutveckling
ID : 20170059
Pays : International
Organisme : Vetenskapsrådet
ID : 2014-4573
Pays : International
Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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