Expression, Purification, and Activity Analysis of Chlorophyllide Oxidoreductase and Ni
Chlorophyll biosynthesis
Chlorophyllide oxidoreductase (COR)
Coenzyme F430 biosynthesis
Dynamic switch protein
Ni2+-sirohydrochlorin a,c-diamide reductase (CfbC/D)
Nitrogenase-like enzymes
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
15
10
2018
pubmed:
15
10
2018
medline:
14
6
2019
Statut:
ppublish
Résumé
Nitrogenase-like enzymes play a vital role in the reduction of the conjugated ring systems of diverse tetrapyrrole molecules. The biosynthesis of all bacteriochlorophylls involves the two-electron reduction of the C7-C8 double bond of the green pigment chlorophyllide, which is catalyzed by the nitrogenase-like two-component metalloenzyme chlorophyllide oxidoreductase (COR); whereas in all methanogenic microbes, another nitrogenase-like system, CfbC/D, is responsible for the sophisticated six-electron reduction of Ni
Identifiants
pubmed: 30317478
doi: 10.1007/978-1-4939-8864-8_8
doi:
Substances chimiques
Metalloporphyrins
0
Metalloproteins
0
Multienzyme Complexes
0
Uroporphyrins
0
Chlorophyll
1406-65-1
sirohydrochlorin
65207-12-7
factor F430
73145-13-8
Nickel
7OV03QG267
Alcohol Oxidoreductases
EC 1.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM