3D Chemical Shift-Encoded MRI for Volume and Composition Quantification of Abdominal Adipose Tissue During an Overfeeding Protocol in Healthy Volunteers.
Abdominal Fat
/ diagnostic imaging
Adipose Tissue
/ diagnostic imaging
Adolescent
Adult
Biopsy, Needle
Body Weight
Diet
Gas Chromatography-Mass Spectrometry
Healthy Volunteers
Humans
Image Processing, Computer-Assisted
/ methods
Imaging, Three-Dimensional
/ methods
Intra-Abdominal Fat
/ diagnostic imaging
Liver
/ diagnostic imaging
Longitudinal Studies
Magnetic Resonance Imaging
/ methods
Magnetic Resonance Spectroscopy
Male
Middle Aged
Overweight
/ diagnostic imaging
Prospective Studies
Spectrophotometry
Young Adult
MR spectroscopy
chemical shift-encoded imaging
fatty acid composition
gas chromatography-mass spectrometry
in vivo
overfeeding
Journal
Journal of magnetic resonance imaging : JMRI
ISSN: 1522-2586
Titre abrégé: J Magn Reson Imaging
Pays: United States
ID NLM: 9105850
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
25
07
2018
revised:
14
09
2018
accepted:
14
09
2018
pubmed:
18
10
2018
medline:
7
10
2020
entrez:
18
10
2018
Statut:
ppublish
Résumé
Overweight and obesity are major worldwide health concerns characterized by an abnormal accumulation of fat in adipose tissue (AT) and liver. To evaluate the volume and the fatty acid (FA) composition of the subcutaneous adipose tissue (SAT) and the visceral adipose tissue (VAT) and the fat content in the liver from 3D chemical-shift-encoded (CSE)-MRI acquisition, before and after a 31-day overfeeding protocol. Prospective and longitudinal study. Twenty-one nonobese healthy male volunteers. A 3D spoiled-gradient multiple echo sequence and STEAM sequence were performed at 3T. AT volume was automatically segmented on CSE-MRI between L2 to L4 lumbar vertebrae and compared to the dual-energy X-ray absorptiometry (DEXA) measurement. CSE-MRI and MR spectroscopy (MRS) data were analyzed to assess the proton density fat fraction (PDFF) in the liver and the FA composition in SAT and VAT. Gas chromatography-mass spectrometry (GC-MS) analyses were performed on 13 SAT samples as a FA composition countermeasure. Paired t-test, Pearson's correlation coefficient, and Bland-Altman plots were used to compare measurements. SAT and VAT volumes significantly increased (P < 0.001). CSE-MRI and DEXA measurements were strongly correlated (r = 0.98, P < 0.001). PDFF significantly increased in the liver (+1.35, P = 0.002 for CSE-MRI, + 1.74, P = 0.002 for MRS). FA composition of SAT and VAT appeared to be consistent between localized-MRS and CSE-MRI (on whole segmented volume) measurements. A significant difference between SAT and VAT FA composition was found (P < 0.001 for CSE-MRI, P = 0.001 for MRS). MRS and CSE-MRI measurements of the FA composition were correlated with the GC-MS results (for ndb: r 1 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2019;49:1587-1599.
Sections du résumé
BACKGROUND
Overweight and obesity are major worldwide health concerns characterized by an abnormal accumulation of fat in adipose tissue (AT) and liver.
PURPOSE
To evaluate the volume and the fatty acid (FA) composition of the subcutaneous adipose tissue (SAT) and the visceral adipose tissue (VAT) and the fat content in the liver from 3D chemical-shift-encoded (CSE)-MRI acquisition, before and after a 31-day overfeeding protocol.
STUDY TYPE
Prospective and longitudinal study.
SUBJECTS
Twenty-one nonobese healthy male volunteers.
FIELD STRENGTH/SEQUENCE
A 3D spoiled-gradient multiple echo sequence and STEAM sequence were performed at 3T.
ASSESSMENT
AT volume was automatically segmented on CSE-MRI between L2 to L4 lumbar vertebrae and compared to the dual-energy X-ray absorptiometry (DEXA) measurement. CSE-MRI and MR spectroscopy (MRS) data were analyzed to assess the proton density fat fraction (PDFF) in the liver and the FA composition in SAT and VAT. Gas chromatography-mass spectrometry (GC-MS) analyses were performed on 13 SAT samples as a FA composition countermeasure.
STATISTICAL TESTS
Paired t-test, Pearson's correlation coefficient, and Bland-Altman plots were used to compare measurements.
RESULTS
SAT and VAT volumes significantly increased (P < 0.001). CSE-MRI and DEXA measurements were strongly correlated (r = 0.98, P < 0.001). PDFF significantly increased in the liver (+1.35, P = 0.002 for CSE-MRI, + 1.74, P = 0.002 for MRS). FA composition of SAT and VAT appeared to be consistent between localized-MRS and CSE-MRI (on whole segmented volume) measurements. A significant difference between SAT and VAT FA composition was found (P < 0.001 for CSE-MRI, P = 0.001 for MRS). MRS and CSE-MRI measurements of the FA composition were correlated with the GC-MS results (for ndb: r
LEVEL OF EVIDENCE
1 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2019;49:1587-1599.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1587-1599Subventions
Organisme : LABEX PRIMES
ID : ANR-11-LABX-0063
Pays : International
Organisme : Université de Lyon
Pays : International
Organisme : "Investissements d'Avenir" program
ID : ANR-11-IDEX-0007
Pays : International
Organisme : French National Research Agency (ANR)
Pays : International
Organisme : IHU OpéRa
ID : ANR-10-IBHU-0004
Pays : International
Informations de copyright
© 2018 International Society for Magnetic Resonance in Medicine.