Resveratrol attenuates oxidative stress during experimental periodontitis in rats exposed to cigarette smoke inhalation.
Animals
Anti-Inflammatory Agents, Non-Steroidal
/ pharmacology
Antioxidants
/ pharmacology
Cigarette Smoking
/ adverse effects
Enzyme-Linked Immunosorbent Assay
Gingiva
/ metabolism
Male
Mandible
Molar
NADP
/ metabolism
Oxidative Stress
/ drug effects
Periodontitis
/ metabolism
Polymerase Chain Reaction
RNA, Messenger
/ metabolism
Rats, Wistar
Resveratrol
/ pharmacology
Sirtuin 1
/ genetics
Superoxide Dismutase-1
/ metabolism
oxidative stress
periodontitis
resveratrol
smoking
Journal
Journal of periodontal research
ISSN: 1600-0765
Titre abrégé: J Periodontal Res
Pays: United States
ID NLM: 0055107
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
29
05
2018
revised:
17
08
2018
accepted:
12
09
2018
pubmed:
23
10
2018
medline:
19
9
2019
entrez:
23
10
2018
Statut:
ppublish
Résumé
This study aimed at investigating the effect of the systemic administration of resveratrol (RESV) on oxidative stress during experimental periodontitis in rats subjected to cigarette smoke inhalation. Experimental periodontitis (EP) was induced in 26 male Wistar rats by the insertion of a ligature around one of the first mandibular and maxillary molars. The animals were assigned randomly to the following groups: cigarette smoke inhalation (CSI; 3 times/d, 8 minutes/d) + resveratrol (10 mg/Kg), that is, SMK + RESV (n = 13) and cigarette smoke inhalation + placebo, that is, SMK + PLAC (n = 13). The substances were administered daily for 30 days (19 days prior and 11 days following EP induction), and then, the animals were euthanized. The maxillary specimens were processed for morphometric analysis of bone loss, and the tissue surrounding the first maxillary molars was collected for mRNA quantification of Sirtuin 1 (SIRT1) by real-time PCR. The gingival tissues surrounding the mandibular first molars were collected for quantification of superoxide dismutase 1 (SOD1) and nicotinamide adenine dinucleotide phosphatase oxidase (NADPH) using an ELISA assay. Reduced bone loss was demonstrated in animals in the SMK + RESV group as compared to those in the SMK + PLAC (P < 0.05) group on the basis of morphometric analysis. Resveratrol promoted higher levels of SIRT and SOD (P < 0.05) as well as reduced levels of NADPH oxidase (P < 0.05) were found in tissues derived from animals in the SMK + RESV group when compared to those in the SMK + PLAC group. Resveratrol is an efficient therapeutic agent that reduces exacerbation of bone loss found in animals with EP that were also exposed to smoke. The results suggest that its effects could be mediated, at least in part, by its antioxidant and anti-inflammatory properties which attenuate the effects of oxidative stress on EP in the presence of cigarette smoke.
Substances chimiques
Anti-Inflammatory Agents, Non-Steroidal
0
Antioxidants
0
RNA, Messenger
0
NADP
53-59-8
Sod1 protein, rat
EC 1.15.1.1
Superoxide Dismutase-1
EC 1.15.1.1
Sirt1 protein, rat
EC 3.5.1.-
Sirtuin 1
EC 3.5.1.-
Resveratrol
Q369O8926L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
225-232Subventions
Organisme : São Paulo Research Foundation (FAPESP)
ID : 2014/09480-0
Organisme : São Paulo Research Foundation (FAPESP)
ID : 2015/13894-5
Organisme : National Counsel of Technological and Scientific Development (CNPq)
ID : 308045/2014-9
Informations de copyright
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.