Effect of inactivation of luxS gene on the properties of Serratia proteamaculans 94 strain.
Bacterial Proteins
/ genetics
Biofilms
/ growth & development
Carbon-Sulfur Lyases
/ genetics
Gene Expression Regulation, Bacterial
Gene Silencing
Homoserine
/ analogs & derivatives
Lactones
/ metabolism
Meat
/ microbiology
Microbial Interactions
Phenotype
Quorum Sensing
/ genetics
Serratia
/ genetics
Volatile Organic Compounds
/ analysis
Journal
Folia microbiologica
ISSN: 1874-9356
Titre abrégé: Folia Microbiol (Praha)
Pays: United States
ID NLM: 0376757
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
27
04
2018
accepted:
12
10
2018
pubmed:
27
10
2018
medline:
19
9
2019
entrez:
27
10
2018
Statut:
ppublish
Résumé
The luxS gene is responsible for the synthesis of AI-2 (autoinducer-2), a signaling molecule that participates in quorum sensing regulation in a large number of bacteria. In this work, we investigated which phenotypes are regulated by luxS gene in Serratia proteamaculans 94, psychrotrophic strain isolated from spoiled refrigerated meat. AI-2 was identified in S. proteamaculans 94, and the luxS gene involved in its synthesis was cloned and sequenced. A mutant with the inactivated luxS gene was constructed. Inactivation of the luxS gene was shown to lead to the absence of AI-2 synthesis, chitinolytic activity, swimming motility, suppression of the growth of fungal plant pathogens Rhizoctonia solani and Helminthosporium sativum by volatile compounds emitted by S. proteamaculans 94 strain, and to a decrease of extracellular proteolytic activity. The knockout of the luxS gene did not affect synthesis of N-acyl-homoserine lactones, lipolytic, and hemolytic activities of S. proteamaculans 94.
Identifiants
pubmed: 30361877
doi: 10.1007/s12223-018-0657-5
pii: 10.1007/s12223-018-0657-5
doi:
Substances chimiques
Bacterial Proteins
0
Lactones
0
N-octanoylhomoserine lactone
0
Volatile Organic Compounds
0
Homoserine
6KA95X0IVO
Carbon-Sulfur Lyases
EC 4.4.-
LuxS protein, Bacteria
EC 4.4.1.21
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
265-272Subventions
Organisme : Russian Foundation for Basic Research
ID : 16-04-01048
Organisme : Russian Foundation for Basic Research
ID : 18-04-00375
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