Menthol as an effective inhibitor of quorum sensing and biofilm formation in Candida albicans and Candida glabrata by targeting the transcriptional repressor TUP1.
Biofilms
/ drug effects
Candida albicans
/ drug effects
Quorum Sensing
/ drug effects
Candida glabrata
/ drug effects
Menthol
/ pharmacology
Antifungal Agents
/ pharmacology
Reactive Oxygen Species
/ metabolism
Fungal Proteins
/ metabolism
Repressor Proteins
/ metabolism
Gene Expression Regulation, Fungal
/ drug effects
Virulence Factors
/ genetics
Microbial Sensitivity Tests
Candida albicans
Candida glabrata
Biofilm
Gene expression
Menthol
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
01 Nov 2024
01 Nov 2024
Historique:
received:
06
09
2024
accepted:
23
10
2024
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
epublish
Résumé
Menthol, a natural quorum sensing molecule, is derived from the Mentha species. Combating pathogenicity by inactivating quorum sensing is an emerging approach. Therefore, our objective was to investigate anti-quorum sensing and anti-biofilm potentials of menthol in Candida albicans and Candida glabrata. The antifungal properties of menthol were evaluated using a broth microdilution assay and a time-kill assay, and its effects on quorum sensing-mediated virulence factors, cellular reactive oxygen species (ROS), and biofilm formation were tested by evaluating TUP1 expression levels in both C. albicans and C. glabrata. Quorum sensing-mediated virulence factors and biofilm formation were inhibited by menthol in both C. albicans and C. glabrata. Furthermore, coinciding with elevated ROS levels, mRNAs of the quorum sensing-related gene TUP1 were upregulated in both C. albicans and C. glabrata. This study highlights the anti-quorum sensing potential of menthol through the inhibition of quorum sensing-mediated virulence factors, ROS generation, and biofilm development by targeting TUP1, which could have potential in the treatment of Candida infections.
Sections du résumé
BACKGROUND
BACKGROUND
Menthol, a natural quorum sensing molecule, is derived from the Mentha species. Combating pathogenicity by inactivating quorum sensing is an emerging approach. Therefore, our objective was to investigate anti-quorum sensing and anti-biofilm potentials of menthol in Candida albicans and Candida glabrata.
METHODS
METHODS
The antifungal properties of menthol were evaluated using a broth microdilution assay and a time-kill assay, and its effects on quorum sensing-mediated virulence factors, cellular reactive oxygen species (ROS), and biofilm formation were tested by evaluating TUP1 expression levels in both C. albicans and C. glabrata.
RESULTS
RESULTS
Quorum sensing-mediated virulence factors and biofilm formation were inhibited by menthol in both C. albicans and C. glabrata. Furthermore, coinciding with elevated ROS levels, mRNAs of the quorum sensing-related gene TUP1 were upregulated in both C. albicans and C. glabrata.
CONCLUSIONS
CONCLUSIONS
This study highlights the anti-quorum sensing potential of menthol through the inhibition of quorum sensing-mediated virulence factors, ROS generation, and biofilm development by targeting TUP1, which could have potential in the treatment of Candida infections.
Identifiants
pubmed: 39485542
doi: 10.1007/s11033-024-10054-1
pii: 10.1007/s11033-024-10054-1
doi:
Substances chimiques
Menthol
1490-04-6
Antifungal Agents
0
Reactive Oxygen Species
0
Fungal Proteins
0
Repressor Proteins
0
Virulence Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1114Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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