Collection of Phloem Sap in Phytoplasma-Infected Plants.
Aphid
Ca2+
EDTA
Exudation
Phloem sap
Sieve elements
Stylectomie
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
27
10
2018
pubmed:
27
10
2018
medline:
14
6
2019
Statut:
ppublish
Résumé
Phytoplasmas colonize specifically the phloem sieve elements (SEs) of plants and influence effectively the plant physiology. To study and understand the interaction of phytoplasmas and host plants an access to the cellular, microscale volume of SEs is demanded. Different methods are suitable to collect phloem sap of phytoplasma-infected plants. The two most common methods are the EDTA-facilitated exudation and the stylectomy. For the EDTA-facilitated method, the cut end of a leaf is placed into an EDTA solution. The EDTA prevents and avoids the Ca
Identifiants
pubmed: 30362011
doi: 10.1007/978-1-4939-8837-2_21
doi:
Substances chimiques
Edetic Acid
9G34HU7RV0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM