Preparation of a stable and robust nanobiocatalyst by efficiently immobilizing of pectinase onto cyanuric chloride-functionalized chitosan grafted magnetic nanoparticles.
Chitosan
Conformational stability
Cyanuric chloride
Magnetic nanoparticles
Pectinase immobilization
Reusability
Journal
Journal of colloid and interface science
ISSN: 1095-7103
Titre abrégé: J Colloid Interface Sci
Pays: United States
ID NLM: 0043125
Informations de publication
Date de publication:
15 Feb 2019
15 Feb 2019
Historique:
received:
07
08
2018
revised:
12
10
2018
accepted:
18
10
2018
pubmed:
28
10
2018
medline:
22
1
2019
entrez:
28
10
2018
Statut:
ppublish
Résumé
In industrial processes, effective degradation of polygalacturonic acid using immobilized pectinase is preferred over free one due to its stability and efficient functional reuses. Pectinase was covalently conjugated to the surface of cyanuric chloride functionalized chitosan encapsulated magnetite nanoparticles. The results obtained of various analytical tools and biochemical studies demonstrated successful synthesis and immobilization processes, high immobilization efficiency and loading capacity. The circular dichroism (CD) results of free and immobilized pectinase revealed the partial decreases in the α-helices and β-sheets, and marginal increases in the unordered elements contents of pectinase upon the immobilization onto Fe
Identifiants
pubmed: 30368098
pii: S0021-9797(18)31250-5
doi: 10.1016/j.jcis.2018.10.053
pii:
doi:
Substances chimiques
Enzymes, Immobilized
0
Magnetite Nanoparticles
0
Triazines
0
cyanuric chloride
5U4L4QHD6I
Chitosan
9012-76-4
Polygalacturonase
EC 3.2.1.15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
261-270Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.