Superactive β-galactosidase inclusion bodies.

Cloning, Molecular Enzyme Stability Escherichia coli / enzymology Escherichia coli Proteins / chemistry Gene Expression Genetic Vectors / chemistry Hot Temperature Hydrogen-Ion Concentration Inclusion Bodies / chemistry Kinetics Lactose / chemistry Protein Aggregates Recombinant Proteins / chemistry Solubility Structure-Activity Relationship beta-Galactosidase / chemistry
Catalytic activity Inclusion bodies Protein desorption process β-Galactosidase

Journal

Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133

Informations de publication

Date de publication:
01 Jan 2019
Historique:
received: 19 07 2018
revised: 23 09 2018
accepted: 17 10 2018
pubmed: 2 11 2018
medline: 22 3 2019
entrez: 2 11 2018
Statut: ppublish

Résumé

Bacterial inclusion bodies (IBs) were historically considered one of the major obstacles in protein production through recombinant DNA techniques and conceived as amorphous deposits formed by passive and rather unspecific structures of unfolded proteins aggregates. Subsequent studies demonstrated that IBs contained an important quantity of active protein. In this work, we proved that recombinant β-galactosidase inclusion bodies (IB

Identifiants

DOI: 10.1016/j.colsurfb.2018.10.049 PMID: 30384274
pubmed: 30384274
pii: S0927-7765(18)30746-X
doi: 10.1016/j.colsurfb.2018.10.049
pii:
doi:

Substances chimiques

Escherichia coli Proteins 0
Protein Aggregates 0
Recombinant Proteins 0
beta-Galactosidase EC 3.2.1.23
Lactose J2B2A4N98G

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

769-775

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Sandra S Flores (SS)

Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, ICTA and Departamento de Química, Cátedra de Química Biológica, Córdoba, Argentina; CONICET, Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT), Córdoba, Argentina.

Verónica Nolan (V)

Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, ICTA and Departamento de Química, Cátedra de Química Biológica, Córdoba, Argentina; CONICET, Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT), Córdoba, Argentina.

María A Perillo (MA)

Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, ICTA and Departamento de Química, Cátedra de Química Biológica, Córdoba, Argentina; CONICET, Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT), Córdoba, Argentina.

Julieta M Sánchez (JM)

Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, ICTA and Departamento de Química, Cátedra de Química Biológica, Córdoba, Argentina; CONICET, Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT), Córdoba, Argentina. Electronic address: julieta.sanchez@unc.edu.ar.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Vecteurs génétiques › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Température élevée › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Cellules › Structures cellulaires › Corps d'inclusion › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Glucides › Sucres › Diholoside › Lactose › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Structures macromoléculaires › Agrégats de protéines › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Phénomènes chimiques › Solubilité › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Relation structure-activité › Superactive β-galactosidase inclusion bodies.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Galactosidases › beta-Galactosidase › Superactive β-galactosidase inclusion bodies.