One step open fermentation for lactic acid production from inedible starchy biomass by thermophilic Bacillus coagulans IPE22.
Bacillus coagulans
Inedible starchy biomass
Lactic acid
Simultaneous liquefaction saccharification and fermentation
l-lactate dehydrogenase
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
30
09
2018
revised:
18
10
2018
accepted:
19
10
2018
pubmed:
6
11
2018
medline:
14
8
2019
entrez:
3
11
2018
Statut:
ppublish
Résumé
The aim of this study was to establish a simplified operational process for lactic acid (LA) production by Bacillus coagulans IPE22 from inedible starchy biomass with open fermentation method. First, 29.47 mU/mg specific amylase activity was detected in direct batch fermentation from soluble starch, but the activity of the produced amylase was too low for effective production of LA. Then seven batches from 72 g/L soluble starch were conducted without sterilization. It was found that one step simultaneous liquefaction, saccharification and fermentation (SLSF) with the addition of mesothermal α-amylase and glucoamylase was the optimal mode with LA concentration, yield and productivity of 68.72 g/L, 0.99 g/g and 1.72 g/L h respectively. Finally, inedible starchy biomass, cassava and sorghum flours, were proved to be alternatives to refined soluble starch. For the first time, one step open SLSF of inedible starchy biomass was reported for LA production by B. coagulans.
Identifiants
pubmed: 30388577
pii: S0960-8524(18)31474-3
doi: 10.1016/j.biortech.2018.10.043
pii:
doi:
Substances chimiques
Lactic Acid
33X04XA5AT
Starch
9005-25-8
Glucan 1,4-alpha-Glucosidase
EC 3.2.1.3
Types de publication
Journal Article
Langues
eng
Pagination
398-406Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.