Processive flow by biased polymerization mediates the slow axonal transport of actin.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
07 01 2019
07 01 2019
Historique:
received:
04
11
2017
revised:
02
09
2018
accepted:
25
10
2018
pubmed:
8
11
2018
medline:
23
10
2019
entrez:
8
11
2018
Statut:
ppublish
Résumé
Classic pulse-chase studies have shown that actin is conveyed in slow axonal transport, but the mechanistic basis for this movement is unknown. Recently, we reported that axonal actin was surprisingly dynamic, with focal assembly/disassembly events ("actin hotspots") and elongating polymers along the axon shaft ("actin trails"). Using a combination of live imaging, superresolution microscopy, and modeling, in this study, we explore how these dynamic structures can lead to processive transport of actin. We found relatively more actin trails elongated anterogradely as well as an overall slow, anterogradely biased flow of actin in axon shafts. Starting with first principles of monomer/filament assembly and incorporating imaging data, we generated a quantitative model simulating axonal hotspots and trails. Our simulations predict that the axonal actin dynamics indeed lead to a slow anterogradely biased flow of the population. Collectively, the data point to a surprising scenario where local assembly and biased polymerization generate the slow axonal transport of actin without involvement of microtubules (MTs) or MT-based motors. Mechanistically distinct from polymer sliding, this might be a general strategy to convey highly dynamic cytoskeletal cargoes.
Identifiants
pubmed: 30401699
pii: jcb.201711022
doi: 10.1083/jcb.201711022
pmc: PMC6314539
doi:
Substances chimiques
Actins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112-124Subventions
Organisme : NIA NIH HHS
ID : P50 AG033514
Pays : United States
Informations de copyright
© 2018 Chakrabarty et al.
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