Seeing and sensing single G protein-coupled receptors by atomic force microscopy.
Journal
Current opinion in cell biology
ISSN: 1879-0410
Titre abrégé: Curr Opin Cell Biol
Pays: England
ID NLM: 8913428
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
02
10
2018
revised:
23
10
2018
accepted:
23
10
2018
pubmed:
10
11
2018
medline:
18
1
2020
entrez:
10
11
2018
Statut:
ppublish
Résumé
G protein-coupled receptors (GPCRs) relay extracellular information across cell membranes through a continuum of conformations that are not always captured in structures. Hence, complementary approaches are required to quantify the physical and chemical properties of the dynamic conformations linking to GPCR function. Atomic force microscopy (AFM)-based high-resolution imaging and force spectroscopy are unique methods to scrutinize GPCRs and to sense their interactions. Here, we exemplify recent AFM-based applications to directly observe the supramolecular assembly of GPCRs in native membranes, to measure the ligand-binding free-energy landscape, and how interactions modulate the structural properties of GPCRs. Common trends in GPCR function are beginning to emerge. We envision that technical developments in combining AFM with superresolution fluorescence imaging will provide insights into how cellular states modulate GPCRs and vice versa.
Identifiants
pubmed: 30412846
pii: S0955-0674(18)30072-3
doi: 10.1016/j.ceb.2018.10.006
pmc: PMC6472649
pii:
doi:
Substances chimiques
Ligands
0
Receptors, G-Protein-Coupled
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
25-32Informations de copyright
Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.
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