David Alsteens

Affiliations

Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.

Publications (44)

Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy.

Nano letters

Avec: Melanie Koehler , Cristina Lo Giudice , Philipp Vogl , Andreas Ebner , Peter Hinterdorfer , Hermann J Gruber , David Alsteens

Reovirus directly engages integrin to recruit clathrin for entry into host cells.

Nature communications

Avec: Melanie Koehler , Simon J L Petitjean , Jinsung Yang , Pavithra Aravamudhan , Xayathed Somoulay , Cristina Lo Giudice , Mégane A Poncin , Andra C Dumitru , Terence S Dermody , David Alsteens

In-Depth Analysis of the Pancreatic Extracellular Matrix during Development for Next-Generation Tissue Engineering.

International journal of molecular sciences

Avec: Laura Glorieux , Laura Vandooren , Sylvie Derclaye , Sébastien Pyr Dit Ruys , Paloma Oncina-Gil , Anna Salowka , Gaëtan Herinckx , Elias Aajja , Pascale Lemoine , Catherine Spourquet , Hélène Lefort , Patrick Henriet , Donatienne Tyteca , Francesca M Spagnoli , David Alsteens , Didier Vertommen , Christophe E Pierreux

Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus.

Nature communications

Avec: Pengcheng Shang , Joshua D Simpson , Gwen M Taylor , Danica M Sutherland , Olivia L Welsh , Pavithra Aravamudhan , Rita Dos Santos Natividade , Kristina Schwab , Joshua J Michel , Amanda C Poholek , Yijen Wu , Dhivyaa Rajasundaram , Melanie Koehler , David Alsteens , Terence S Dermody

Single-Molecule Investigation of the Binding Interface Stability of SARS-CoV-2 Variants with ACE2.

ACS nanoscience Au

Avec: Ankita Ray , Thu Thi Minh Tran , Rita Dos Santos Natividade , Rodrigo A Moreira , Joshua D Simpson , Danahe Mohammed , Melanie Koehler , Simon J L Petitjean , Qingrong Zhang , Fabrice Bureau , Laurent Gillet , Adolfo B Poma , David Alsteens

The

iScience

Avec: Laurence Lecordier , Sophie Uzureau , Gilles Vanwalleghem , Magali Deleu , Jean-Marc Crowet , Paul Barry , Barry Moran , Paul Voorheis , Andra-Cristina Dumitru , Yoshiki Yamaryo-Botté , Marc Dieu , Patricia Tebabi , Benoit Vanhollebeke , Laurence Lins , Cyrille Y Botté , David Alsteens , Yves Dufrêne , David Pérez-Morga , Derek P Nolan , Etienne Pays

Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans.

Annual review of virology

Avec: Melanie Koehler , Martin Delguste , Christian Sieben , Laurent Gillet , David Alsteens

Mechanical Forces between Mycobacterial Antigen 85 Complex and Fibronectin.

Cells

Avec: Albertus Viljoen , David Alsteens , Yves Dufrêne

Altered Glycan Expression on Breast Cancer Cells Facilitates Infection by T3 Seroptype Oncolytic Reovirus.

Nano letters

Avec: Danahe Mohammed , Melanie Koehler , Andra C Dumitru , Pavithra Aravamudhan , Danica M Sutherland , Terence S Dermody , David Alsteens

Force-clamp spectroscopy identifies a catch bond mechanism in a Gram-positive pathogen.

Nature communications

Avec: Marion Mathelié-Guinlet , Felipe Viela , Giampiero Pietrocola , Pietro Speziale , David Alsteens , Yves F Dufrêne

Stepwise Enzymatic-Dependent Mechanism of Ebola Virus Binding to Cell Surface Receptors Monitored by AFM.

Nano letters

Avec: Qingrong Zhang , Jinsung Yang , Sueli Tillieux , Zhengyuan Guo , Rita Dos Santos Natividade , Melanie Koehler , Simon Petitjean , Zongqiang Cui , David Alsteens

Deciphering molecular mechanisms stabilizing the reovirus-binding complex.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Rita Dos Santos Natividade , Melanie Koehler , Priscila S F C Gomes , Joshua D Simpson , Sydni Caet Smith , Diego E B Gomes , Juliette de Lhoneux , Jinsung Yang , Ankita Ray , Terence S Dermody , Rafael C Bernardi , Kristen M Ogden , David Alsteens

Liquid-Liquid Phase Separation Enhances TDP-43 LCD Aggregation but Delays Seeded Aggregation.

Biomolecules

Avec: Donya Pakravan , Emiel Michiels , Anna Bratek-Skicki , Mathias De Decker , Joris Van Lindt , David Alsteens , Sylvie Derclaye , Philip Van Damme , Joost Schymkowitz , Frederic Rousseau , Peter Tompa , Ludo Van Den Bosch

Label-Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Avec: Andra C Dumitru , Danahe Mohammed , Mauriane Maja , Jinsung Yang , Sandrine Verstraeten , Aranzazu Del Campo , Marie-Paule Mingeot-Leclercq , Donatienne Tyteca , David Alsteens

Probing ligand-receptor bonds in physiologically relevant conditions using AFM.

Analytical and bioanalytical chemistry

Avec: Cristina Lo Giudice , Andra C Dumitru , David Alsteens

Glycan-mediated enhancement of reovirus receptor binding.

Nature communications

Avec: Melanie Koehler , Pavithra Aravamudhan , Camila Guzman-Cardozo , Andra C Dumitru , Jinsung Yang , Serena Gargiulo , Patrice Soumillion , Terence S Dermody , David Alsteens

Impaired Cytoskeletal and Membrane Biophysical Properties of Acanthocytes in Hypobetalipoproteinemia - A Case Study.

Frontiers in physiology

Avec: Anne-Sophie Cloos , Laura G M Daenen , Mauriane Maja , Amaury Stommen , Juliette Vanderroost , Patrick Van Der Smissen , Minke Rab , Jan Westerink , Eric Mignolet , Yvan Larondelle , Romano Terrasi , Giulio G Muccioli , Andra C Dumitru , David Alsteens , Richard van Wijk , Donatienne Tyteca

Probing PIEZO1 Localization upon Activation Using High-Resolution Atomic Force and Confocal Microscopy.

Nano letters

Avec: Andra C Dumitru , Amaury Stommen , Melanie Koehler , Anne-Sophie Cloos , Jinsung Yang , Arnaud Leclercqz , Donatienne Tyteca , David Alsteens

NRP1 is a receptor for mammalian orthoreovirus engaged by distinct capsid subunits.

Cell host & microbe

Avec: Pengcheng Shang , Rita Dos Santos Natividade , Gwen M Taylor , Ankita Ray , Olivia L Welsh , Kay L Fiske , Danica M Sutherland , David Alsteens , Terence S Dermody

Single-Molecule Analysis of SARS-CoV-2 Binding to C-Type Lectin Receptors.

Nano letters

Avec: Joshua D Simpson , Ankita Ray , Claire Marcon , Rita Dos Santos Natividade , Gol Mohammad Dorrazehi , Kimberly Durlet , Melanie Koehler , David Alsteens

Mechanochemical Activation of Class-B G-Protein-Coupled Receptor upon Peptide-Ligand Binding.

Nano letters

Avec: Cristina Lo Giudice , Haonan Zhang , Beili Wu , David Alsteens

Multiparametric Atomic Force Microscopy Identifies Multiple Structural and Physical Heterogeneities on the Surface of

ACS omega

Avec: Claire Valotteau , Andra C Dumitru , Laurence Lecordier , David Alsteens , Etienne Pays , David Pérez-Morga , Yves F Dufrêne

Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166.

Nature communications

Avec: Henri-François Renard , François Tyckaert , Cristina Lo Giudice , Thibault Hirsch , Cesar Augusto Valades-Cruz , Camille Lemaigre , Massiullah Shafaq-Zadah , Christian Wunder , Ruddy Wattiez , Ludger Johannes , Pierre van der Bruggen , David Alsteens , Pierre Morsomme

Molecular insights into receptor binding energetics and neutralization of SARS-CoV-2 variants.

Nature communications

Avec: Melanie Koehler , Ankita Ray , Rodrigo A Moreira , Blinera Juniku , Adolfo B Poma , David Alsteens

Stress-Induced Catch-Bonds to Enhance Bacterial Adhesion.

Trends in microbiology

Avec: Marion Mathelié-Guinlet , Felipe Viela , David Alsteens , Yves F Dufrêne

Atomic Force Microscopy-Based Force Spectroscopy and Multiparametric Imaging of Biomolecular and Cellular Systems.

Chemical reviews

Avec: Daniel J Müller , Andra C Dumitru , Cristina Lo Giudice , Hermann E Gaub , Peter Hinterdorfer , Gerhard Hummer , James J De Yoreo , Yves F Dufrêne , David Alsteens

Surface cholesterol-enriched domains specifically promote invasion of breast cancer cell lines by controlling invadopodia and extracellular matrix degradation.

Cellular and molecular life sciences : CMLS

Avec: Mauriane Maja , Danahe Mohammed , Andra C Dumitru , Sandrine Verstraeten , Maxime Lingurski , Marie-Paule Mingeot-Leclercq , David Alsteens , Donatienne Tyteca

NgR1 binding to reovirus reveals an unusual bivalent interaction and a new viral attachment protein.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Danica M Sutherland , Michael Strebl , Melanie Koehler , Olivia L Welsh , Xinzhe Yu , Liya Hu , Rita Dos Santos Natividade , Jonathan J Knowlton , Gwen M Taylor , Rodolfo A Moreno , Patrick Wörz , Zachery R Lonergan , Pavithra Aravamudhan , Camila Guzman-Cardozo , Sukhleen Kour , Udai Bhan Pandey , David Alsteens , Zhao Wang , B V Venkataram Prasad , Thilo Stehle , Terence S Dermody

Piezo1 Regulation Involves Lipid Domains and the Cytoskeleton and Is Favored by the Stomatocyte-Discocyte-Echinocyte Transformation.

Biomolecules

Avec: Amaury Stommen , Marine Ghodsi , Anne-Sophie Cloos , Louise Conrard , Andra C Dumitru , Patrick Henriet , Christophe E Pierreux , David Alsteens , Donatienne Tyteca

Viral capsid structural assembly governs the reovirus binding interface to NgR1.

Nanoscale horizons

Avec: Rita Dos Santos Natividade , Andra C Dumitru , Alessandro Nicoli , Michael Strebl , Danica M Sutherland , Olivia L Welsh , Mustafa Ghulam , Thilo Stehle , Terence S Dermody , Antonella Di Pizio , Melanie Koehler , David Alsteens

Regulatory Mechanisms of the Mucin-Like Region on Herpes Simplex Virus during Cellular Attachment.

ACS chemical biology

Avec: Martin Delguste , Nadia Peerboom , Grégoire Le Brun , Edward Trybala , Sigvard Olofsson , Tomas Bergström , David Alsteens , Marta Bally

Seeing and sensing single G protein-coupled receptors by atomic force microscopy.

Current opinion in cell biology

Avec: K Tanuj Sapra , Patrizia M Spoerri , Andreas Engel , David Alsteens , Daniel J Müller

Nanophysical Mapping of Inflammasome Activation by Nanoparticles via Specific Cell Surface Recognition Events.

ACS nano

Avec: Cristina Lo Giudice , Jinsung Yang , Mégane A Poncin , Laurent Adumeau , Martin Delguste , Melanie Koehler , Koen Evers , Andra C Dumitru , Kenneth A Dawson , David Alsteens

Role of the Redox State of Human Peroxiredoxin-5 on Its TLR4-Activating DAMP Function.

Antioxidants (Basel, Switzerland)

Avec: Mégane A Poncin , Pierre Van Meerbeeck , Joshua D Simpson , André Clippe , François Tyckaert , Fabrice Bouillenne , Hervé Degand , André Matagne , Pierre Morsomme , Bernard Knoops , David Alsteens

Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor.

Nature communications

Avec: Jinsung Yang , Simon J L Petitjean , Melanie Koehler , Qingrong Zhang , Andra C Dumitru , Wenzhang Chen , Sylvie Derclaye , Stéphane P Vincent , Patrice Soumillion , David Alsteens

Single-Virus Force Spectroscopy Discriminates the Intrinsic Role of Two Viral Glycoproteins upon Cell Surface Attachment.

Nano letters

Avec: Martin Delguste , Grégoire Le Brun , Florian Cotin , Bénédicte Machiels , Laurent Gillet , David Alsteens

Submolecular probing of the complement C5a receptor-ligand binding reveals a cooperative two-site binding mechanism.

Communications biology

Avec: Andra C Dumitru , R N V Krishna Deepak , Heng Liu , Melanie Koehler , Cheng Zhang , Hao Fan , David Alsteens

Reovirus σ1 Conformational Flexibility Modulates the Efficiency of Host Cell Attachment.

Journal of virology

Avec: Julia R Diller , Sean R Halloran , Melanie Koehler , Rita Dos Santos Natividade , David Alsteens , Thilo Stehle , Terence S Dermody , Kristen M Ogden

Atomic force microscopy applied to interrogate nanoscale cellular chemistry and supramolecular bond dynamics for biomedical applications.

Chemical communications (Cambridge, England)

Avec: Joshua D Simpson , Ankita Ray , Melanie Koehler , Danahe Mohammed , David Alsteens

Multivalent 9-O-Acetylated-sialic acid glycoclusters as potent inhibitors for SARS-CoV-2 infection.

Nature communications

Avec: Simon J L Petitjean , Wenzhang Chen , Melanie Koehler , Ravikumar Jimmidi , Jinsung Yang , Danahe Mohammed , Blinera Juniku , Megan L Stanifer , Steeve Boulant , Stéphane P Vincent , David Alsteens

Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signaling.

Science advances

Avec: Benjamin Ledoux , Natacha Zanin , Jinsung Yang , Vincent Mercier , Charlotte Coster , Christine Dupont-Gillain , David Alsteens , Pierre Morsomme , Henri-François Renard

Heparin-Induced Allosteric Changes in SARS-CoV-2 Spike Protein Facilitate ACE2 Binding and Viral Entry.

Nano letters

Avec: Simon J L Petitjean , Savannah Eeckhout , Martin Delguste , Qingrong Zhang , Kimberley Durlet , David Alsteens

AFM-based force spectroscopy unravels stepwise formation of the DNA transposition complex in the widespread Tn3 family mobile genetic elements.

Nucleic acids research

Avec: Maricruz Fernandez , Alexander V Shkumatov , Yun Liu , Claire Stulemeijer , Sylvie Derclaye , Rouslan G Efremov , Bernard Hallet , David Alsteens

Cholesterol and Sphingomyelin Polarize at the Leading Edge of Migrating Myoblasts and Involve Their Clustering in Submicrometric Domains.

Biomolecules

Avec: Juliette Vanderroost , Noémie Avalosse , Danahe Mohammed , Delia Hoffmann , Patrick Henriet , Christophe E Pierreux , David Alsteens , Donatienne Tyteca

Réseau de co-auteurs

Melanie Koehler 20 collaborations

Andra C Dumitru 15 collaborations

Jinsung Yang 10 collaborations

Terence S Dermody 9 collaborations

Rita Dos Santos Natividade 9 collaborations

Cristina Lo Giudice 7 collaborations

Donatienne Tyteca 7 collaborations

Danahe Mohammed 7 collaborations

Joshua D Simpson 6 collaborations

Ankita Ray 6 collaborations

Simon J L Petitjean 5 collaborations

Pavithra Aravamudhan 5 collaborations

Danica M Sutherland 5 collaborations

Martin Delguste 5 collaborations

Sylvie Derclaye 4 collaborations

Olivia L Welsh 4 collaborations

Qingrong Zhang 4 collaborations

Yves F Dufrêne 4 collaborations

Mégane A Poncin 3 collaborations

Patrick Henriet 3 collaborations

Christophe E Pierreux 3 collaborations

Gwen M Taylor 3 collaborations

Laurent Gillet 3 collaborations

Mauriane Maja 3 collaborations

Anne-Sophie Cloos 3 collaborations

Amaury Stommen 3 collaborations

Pierre Morsomme 3 collaborations

Thilo Stehle 3 collaborations

Peter Hinterdorfer 2 collaborations

Pengcheng Shang 2 collaborations

Rodrigo A Moreira 2 collaborations

Adolfo B Poma 2 collaborations

Laurence Lecordier 2 collaborations

Yves Dufrêne 2 collaborations

David Pérez-Morga 2 collaborations

Etienne Pays 2 collaborations

Marion Mathelié-Guinlet 2 collaborations

Felipe Viela 2 collaborations

Kristen M Ogden 2 collaborations

Sandrine Verstraeten 2 collaborations

Marie-Paule Mingeot-Leclercq 2 collaborations

Camila Guzman-Cardozo 2 collaborations

Patrice Soumillion 2 collaborations

Juliette Vanderroost 2 collaborations

Henri-François Renard 2 collaborations

François Tyckaert 2 collaborations

Blinera Juniku 2 collaborations

Daniel J Müller 2 collaborations

Michael Strebl 2 collaborations

Grégoire Le Brun 2 collaborations

Wenzhang Chen 2 collaborations

Stéphane P Vincent 2 collaborations

Philipp Vogl 1 collaboration

Andreas Ebner 1 collaboration

Hermann J Gruber 1 collaboration

Xayathed Somoulay 1 collaboration

Laura Glorieux 1 collaboration

Laura Vandooren 1 collaboration

Sébastien Pyr Dit Ruys 1 collaboration

Paloma Oncina-Gil 1 collaboration

Anna Salowka 1 collaboration

Gaëtan Herinckx 1 collaboration

Elias Aajja 1 collaboration

Pascale Lemoine 1 collaboration

Catherine Spourquet 1 collaboration

Hélène Lefort 1 collaboration

Francesca M Spagnoli 1 collaboration

Didier Vertommen 1 collaboration

Kristina Schwab 1 collaboration

Joshua J Michel 1 collaboration

Amanda C Poholek 1 collaboration

Yijen Wu 1 collaboration

Dhivyaa Rajasundaram 1 collaboration

Thu Thi Minh Tran 1 collaboration

Fabrice Bureau 1 collaboration

Sophie Uzureau 1 collaboration

Gilles Vanwalleghem 1 collaboration

Magali Deleu 1 collaboration

Jean-Marc Crowet 1 collaboration

Paul Barry 1 collaboration

Barry Moran 1 collaboration

Paul Voorheis 1 collaboration

Andra-Cristina Dumitru 1 collaboration

Yoshiki Yamaryo-Botté 1 collaboration

Marc Dieu 1 collaboration

Patricia Tebabi 1 collaboration

Benoit Vanhollebeke 1 collaboration

Laurence Lins 1 collaboration

Cyrille Y Botté 1 collaboration

Derek P Nolan 1 collaboration

Christian Sieben 1 collaboration

Albertus Viljoen 1 collaboration

Giampiero Pietrocola 1 collaboration

Pietro Speziale 1 collaboration

Sueli Tillieux 1 collaboration

Zhengyuan Guo 1 collaboration

Simon Petitjean 1 collaboration

Zongqiang Cui 1 collaboration

Priscila S F C Gomes 1 collaboration

Sydni Caet Smith 1 collaboration

Diego E B Gomes 1 collaboration

Juliette de Lhoneux 1 collaboration

Rafael C Bernardi 1 collaboration

Donya Pakravan 1 collaboration

Emiel Michiels 1 collaboration

Anna Bratek-Skicki 1 collaboration

Mathias De Decker 1 collaboration

Joris Van Lindt 1 collaboration

Philip Van Damme 1 collaboration

Joost Schymkowitz 1 collaboration

Frederic Rousseau 1 collaboration

Peter Tompa 1 collaboration

Ludo Van Den Bosch 1 collaboration

Aranzazu Del Campo 1 collaboration

Serena Gargiulo 1 collaboration

Laura G M Daenen 1 collaboration

Patrick Van Der Smissen 1 collaboration

Minke Rab 1 collaboration

Jan Westerink 1 collaboration

Eric Mignolet 1 collaboration

Yvan Larondelle 1 collaboration

Romano Terrasi 1 collaboration

Giulio G Muccioli 1 collaboration

Richard van Wijk 1 collaboration

Arnaud Leclercqz 1 collaboration

Kay L Fiske 1 collaboration

Claire Marcon 1 collaboration

Gol Mohammad Dorrazehi 1 collaboration

Kimberly Durlet 1 collaboration

Haonan Zhang 1 collaboration

Beili Wu 1 collaboration

Claire Valotteau 1 collaboration

Thibault Hirsch 1 collaboration

Cesar Augusto Valades-Cruz 1 collaboration

Camille Lemaigre 1 collaboration

Massiullah Shafaq-Zadah 1 collaboration

Christian Wunder 1 collaboration

Ruddy Wattiez 1 collaboration

Ludger Johannes 1 collaboration

Pierre van der Bruggen 1 collaboration

Hermann E Gaub 1 collaboration

Gerhard Hummer 1 collaboration

James J De Yoreo 1 collaboration

Maxime Lingurski 1 collaboration

Xinzhe Yu 1 collaboration

Liya Hu 1 collaboration

Jonathan J Knowlton 1 collaboration

Rodolfo A Moreno 1 collaboration

Patrick Wörz 1 collaboration

Zachery R Lonergan 1 collaboration

Sukhleen Kour 1 collaboration

Udai Bhan Pandey 1 collaboration

Zhao Wang 1 collaboration

B V Venkataram Prasad 1 collaboration

Marine Ghodsi 1 collaboration

Louise Conrard 1 collaboration

Alessandro Nicoli 1 collaboration

Mustafa Ghulam 1 collaboration

Antonella Di Pizio 1 collaboration

Nadia Peerboom 1 collaboration

Edward Trybala 1 collaboration

Sigvard Olofsson 1 collaboration

Tomas Bergström 1 collaboration

Marta Bally 1 collaboration

K Tanuj Sapra 1 collaboration

Patrizia M Spoerri 1 collaboration

Andreas Engel 1 collaboration

Laurent Adumeau 1 collaboration

Koen Evers 1 collaboration

Kenneth A Dawson 1 collaboration

Pierre Van Meerbeeck 1 collaboration

André Clippe 1 collaboration

Fabrice Bouillenne 1 collaboration

Hervé Degand 1 collaboration

André Matagne 1 collaboration

Bernard Knoops 1 collaboration

Florian Cotin 1 collaboration

Bénédicte Machiels 1 collaboration

R N V Krishna Deepak 1 collaboration

Heng Liu 1 collaboration

Cheng Zhang 1 collaboration

Hao Fan 1 collaboration

Julia R Diller 1 collaboration

Sean R Halloran 1 collaboration

Ravikumar Jimmidi 1 collaboration

Megan L Stanifer 1 collaboration

Steeve Boulant 1 collaboration

Benjamin Ledoux 1 collaboration

Natacha Zanin 1 collaboration

Vincent Mercier 1 collaboration

Charlotte Coster 1 collaboration

Christine Dupont-Gillain 1 collaboration

Savannah Eeckhout 1 collaboration

Kimberley Durlet 1 collaboration

Maricruz Fernandez 1 collaboration

Alexander V Shkumatov 1 collaboration

Yun Liu 1 collaboration

Claire Stulemeijer 1 collaboration

Rouslan G Efremov 1 collaboration

Bernard Hallet 1 collaboration

Noémie Avalosse 1 collaboration

Delia Hoffmann 1 collaboration