Label-Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells.
atomic force microscopy
cancer cells
cell mechanics
cholesterol
plasma membrane
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
13
07
2020
revised:
24
08
2020
entrez:
26
11
2020
pubmed:
27
11
2020
medline:
27
11
2020
Statut:
epublish
Résumé
Tumor cells present profound alterations in their composition, structural organization, and functional properties. A landmark of cancer cells is an overall altered mechanical phenotype, which so far are linked to changes in their cytoskeletal regulation and organization. Evidence exists that the plasma membrane (PM) of cancer cells also shows drastic changes in its composition and organization. However, biomechanical characterization of PM remains limited mainly due to the difficulties encountered to investigate it in a quantitative and label-free manner. Here, the biomechanical properties of PM of a series of MCF10 cell lines, used as a model of breast cancer progression, are investigated. Notably, a strong correlation between the cell PM elasticity and oncogenesis is observed. The altered membrane composition under cancer progression, as emphasized by the PM-associated cholesterol levels, leads to a stiffening of the PM that is uncoupled from the elastic cytoskeletal properties. Conversely, cholesterol depletion of metastatic cells leads to a softening of their PM, restoring biomechanical properties similar to benign cells. As novel therapies based on targeting membrane lipids in cancer cells represent a promising approach in the field of anticancer drug development, this method contributes to deciphering the functional link between PM lipid content and disease.
Identifiants
pubmed: 33240781
doi: 10.1002/advs.202002643
pii: ADVS2084
pmc: PMC7675049
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2002643Informations de copyright
© 2020 The Authors. Published by Wiley‐VCH GmbH.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Nat Nanotechnol. 2017 Feb;12(2):177-183
pubmed: 27798607
Cancer Res. 2008 Aug 15;68(16):6608-16
pubmed: 18701484
Mol Biol Cell. 2015 May 1;26(9):1699-710
pubmed: 25717184
ACS Biomater Sci Eng. 2019 Aug 12;5(8):3735-3752
pubmed: 33405888
Nat Rev Mol Cell Biol. 2018 May;19(5):281-296
pubmed: 29410529
Biophys J. 2006 Jun 15;90(12):4686-91
pubmed: 16581843
Biophys J. 2009 Jul 22;97(2):674-7
pubmed: 19619482
Cancer Metastasis Rev. 2018 Sep;37(2-3):519-544
pubmed: 29860560
Cell Mol Life Sci. 2015 Dec;72(23):4633-51
pubmed: 26077601
Cancer Metastasis Rev. 2009 Jun;28(1-2):113-27
pubmed: 19153673
Nanoscale Horiz. 2018 May 1;3(3):293-304
pubmed: 32254077
Science. 2016 Apr 15;352(6283):353-8
pubmed: 27013428
Eur Biophys J. 1999;28(4):312-6
pubmed: 10394623
Anal Bioanal Chem. 2019 Oct;411(25):6549-6559
pubmed: 31410537
Front Biosci (Landmark Ed). 2016 Jun 01;21(6):1245-59
pubmed: 27100504
Bioconjug Chem. 2011 Jun 15;22(6):1239-48
pubmed: 21542606
Adv Sci (Weinh). 2020 Oct 08;7(22):2002643
pubmed: 33240781
Cell Death Dis. 2020 Feb 6;11(2):105
pubmed: 32029741
Biophys J. 2004 May;86(5):2965-79
pubmed: 15111412
Biophys J. 2002 May;82(5):2798-810
pubmed: 11964265
Cancer Cell. 2009 Dec 8;16(6):458-62
pubmed: 19962664
Nat Metab. 2020 Feb;2(2):132-141
pubmed: 32694690
Oncogenesis. 2016 Jan 25;5:e189
pubmed: 26807644
Biophys J. 2005 May;88(5):3689-98
pubmed: 15722433
Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):5872-5877
pubmed: 30850523
Sci Rep. 2017 Jul 11;7(1):5117
pubmed: 28698636
Elife. 2015 Aug 14;4:e08905
pubmed: 26274561
Nat Chem. 2018 Nov;10(11):1118-1125
pubmed: 30150727
Nat Commun. 2019 Oct 1;10(1):4460
pubmed: 31575869
Trends Mol Med. 2011 Oct;17(10):564-72
pubmed: 21724466
Rev Sci Instrum. 2014 Jun;85(6):063703
pubmed: 24985823
Eur J Biochem. 2002 Dec;269(24):6195-203
pubmed: 12473115
Angew Chem Int Ed Engl. 2011 Dec 9;50(50):12103-8
pubmed: 22006839
J Clin Oncol. 2009 Mar 10;27(8):1160-7
pubmed: 19204204
Oncotarget. 2017 Sep 19;8(50):88232-88243
pubmed: 29152154
Nat Nanotechnol. 2011 Nov 13;6(12):809-14
pubmed: 22081213
Exp Cell Res. 2004 Jun 10;296(2):151-62
pubmed: 15149846
Nat Commun. 2014 Jul 15;5:4393
pubmed: 25024088
J Cell Biol. 2018 May 7;217(5):1571-1587
pubmed: 29467174
Nature. 2018 Nov;563(7730):203-208
pubmed: 30401836
Clin Exp Metastasis. 2009;26(4):273-87
pubmed: 18498004
ACS Nano. 2019 Aug 27;13(8):9629-9637
pubmed: 31356042
Nat Nanotechnol. 2007 Dec;2(12):780-3
pubmed: 18654431
Nat Phys. 2020 Jan;16(1):101-108
pubmed: 32905405
Biol Cell. 2017 May;109(5):167-189
pubmed: 28244605
J Bacteriol. 2000 Feb;182(4):1172-5
pubmed: 10648548
Mol Cell. 2017 Apr 6;66(1):154-162.e10
pubmed: 28344083
ACS Nano. 2014 Jul 22;8(7):6873-82
pubmed: 24833346
Nat Nanotechnol. 2012 Nov;7(11):757-65
pubmed: 23085644
Chem Commun (Camb). 2018 Jun 19;54(50):6903-6906
pubmed: 29808215
Langmuir. 2012 Dec 11;28(49):16738-44
pubmed: 23198968
ACS Nano. 2016 Mar 22;10(3):3365-74
pubmed: 26901115
Prog Lipid Res. 2012 Jul;51(3):179-98
pubmed: 22342933
Science. 1996 Oct 11;274(5285):255-9
pubmed: 8824192
Proc Natl Acad Sci U S A. 2010 Nov 30;107(48):20744-9
pubmed: 21059927
Trends Cell Biol. 2020 Jul;30(7):556-565
pubmed: 32278656
PLoS One. 2015 May 13;10(5):e0125232
pubmed: 25969993
Nature. 2009 Feb 26;457(7233):1159-62
pubmed: 19098897