High-Throughput Image-Based Screening to Identify Chemical Compounds Capable of Activating FOXO.
Forkhead box O proteins–FOXO
High-content screening
Nucleus-cytoplasm translocation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
11
11
2018
pubmed:
11
11
2018
medline:
6
6
2019
Statut:
ppublish
Résumé
FOXO proteins are transcription factors with important roles in the regulation of the expression of genes involved in cell growth, proliferation, differentiation, and longevity. FOXO proteins are active in the nucleus but, upon post-translational modification they form a docking site for 14-3-3 proteins and are translocated to the cytoplasm where they are inactive.We make use of this regulatory mechanism of FOXO proteins to develop an image-based high-throughput screening platform to detect compounds that regulate FOXO3 subcellular localization. This system has proven a powerful tool to isolate inhibitors of proteins upstream of FOXO, such as PI3K inhibitors.
Identifiants
pubmed: 30414152
doi: 10.1007/978-1-4939-8900-3_13
doi:
Substances chimiques
Forkhead Transcription Factors
0
Recombinant Fusion Proteins
0
Small Molecule Libraries
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM