Initiation of sex change and gonadal gene expression in black sea bass (Centropristis striata) exposed to exemestane, an aromatase inhibitor.
Aromatase
Atresia
Black Sea bass
Estrogen
Exemestane
Hermaphroditism
Protogynous
Sex change
Journal
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
ISSN: 1531-4332
Titre abrégé: Comp Biochem Physiol A Mol Integr Physiol
Pays: United States
ID NLM: 9806096
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
21
09
2018
revised:
24
10
2018
accepted:
30
10
2018
pubmed:
12
11
2018
medline:
1
1
2020
entrez:
12
11
2018
Statut:
ppublish
Résumé
Many teleost fishes exhibit sequential hermaphroditism, where male or female gonads develop first and later undergo sex change. Model sex change species are characterized by social hierarchies and coloration changes, which enable experimental manipulations to better understand these processes. However, other species such as the protogynous black sea bass (Centropristis striata) do not exhibit these characteristics and instead receive research attention due to their importance in fisheries or aquaculture. Black sea bass social structure is unknown, which makes sex change sampling difficult, and few molecular resources are available. The purpose of the present study was to induce sex change using exemestane, an aromatase inhibitor, and assess gonadal gene expression using sex markers (amh, zpc2) and genes involved in steroidogenesis (cyp19a1a, cyp11b), estrogen signaling (esr1, esr2b), and apoptosis or atresia (aen, casp9, fabp11, parg, pdcd4, rif1). Overall, dietary exemestane treatment was effective, and most exposed females exhibited early histological signs of sex change and significantly higher rates of ovarian atresia relative to control females. Genes associated with atresia did not reflect this, however, as expression patterns in sex changing gonads were overall similar to those of ovaries, likely due to a whole ovary dilution effect of the RNA. Still, small but insignificant expression decreases during early sex change were detected for ovary-related genes (aen, casp9, fabp11, zpc2) and anti-apoptotic factors (parg, rif1). Exemestane treatment did not impact spermatogenesis or testicular gene expression, but testes were generally characterized by elevated steroidogenic enzyme and estrogen receptor mRNAs. Further research will be needed to understand these processes in black sea bass, using isolated ovarian follicles and multiple stages of sex change.
Identifiants
pubmed: 30414915
pii: S1095-6433(18)30197-1
doi: 10.1016/j.cbpa.2018.10.024
pmc: PMC6311140
mid: NIHMS1511985
pii:
doi:
Substances chimiques
Androstadienes
0
Aromatase Inhibitors
0
RNA, Messenger
0
exemestane
NY22HMQ4BX
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
51-61Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103423
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103446
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.
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