Ecto-5'-nucleotidase (CD73) is a potential target of hepatocellular carcinoma.
CD73
adenosine
epithelial growth factor receptor (EGFR)
hepatocellular carcinoma (HCC)
knockout mouse
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
16
07
2018
accepted:
09
10
2018
pubmed:
13
11
2018
medline:
10
5
2020
entrez:
13
11
2018
Statut:
ppublish
Résumé
High expression of ecto-5'-nucleotidase (CD73) has been reported in a number of epithelium origin malignancies. Here, we hypothesize that CD73 promotes hepatocellular carcinoma (HCC) growth and metastasis and that the effect is mediated by epithelial growth factor receptor (EGFR). HCC cells with different malignancies and Tissue microarrays of the tumor and peritumoral liver tissues from 30 independent patients were used to examine CD73 and EGFR expression. Then, MTT and Ki67 detection, together with cell adhesion, invasion, and migration assays were used to evaluate the effects of CD73 on cell growth and metastasis. The expression of EGFR in HCC cells was also tested after suppressing or overexpressing CD73. Lastly, tumor tissues from nude mice, which had been injected subcutaneously with HCC cells, were transplanted subcutaneously into CD73
Substances chimiques
GPI-Linked Proteins
0
EGFR protein, human
EC 2.7.10.1
ErbB Receptors
EC 2.7.10.1
5'-Nucleotidase
EC 3.1.3.5
NT5E protein, human
EC 3.1.3.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10248-10259Informations de copyright
© 2018 Wiley Periodicals, Inc.