The roles of flp1 and tadD in Actinobacillus pleuropneumoniae pilus biosynthesis and pathogenicity.

Actinobacillus Infections / blood Actinobacillus pleuropneumoniae / growth & development Animals Bacterial Adhesion / genetics Bacterial Proteins / genetics Biofilms / growth & development Cell Line Disease Models, Animal Female Fimbriae Proteins / genetics Fimbriae, Bacterial / genetics Gene Expression Regulation, Bacterial Genes, Bacterial Homologous Recombination Mice, Inbred BALB C Microbial Viability Phagocytosis Phenotype Sequence Deletion Virulence
Actinobacillus pleuropneumoniae Flp pilus Pathogenicity flp1 tadD

Journal

Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191

Informations de publication

Date de publication:
Jan 2019
Historique:
received: 04 07 2018
revised: 02 11 2018
accepted: 05 11 2018
pubmed: 13 11 2018
medline: 5 3 2019
entrez: 13 11 2018
Statut: ppublish

Résumé

Pili have been demonstrated to contribute to the pathogenicity of many bacterial pathogens. Flp pilus encoded by the tad locus belongs to the type IVb pilus. Our previous study has revealed that the intact tad locus is essential for Flp pilus formation in Actinobacillus pleuropneumoniae, a very important porcine respiratory pathogen. To further investigate the functions of Flp pilus in A. pleuropneumoniae pathogenesis, the flp1 and tadD single deletion mutants were constructed by homologous recombination. Both of the mutant strains lost pilus on their cell surfaces. The abilities of biofilm formation, cell adhesion, resistance to phagocytosis, survival in swine whole blood, and in vivo colonization of the two mutants were significantly reduced compared with those of the parental strain. The corresponding complemented strains recovered the phenotypes. These results demonstrated that flp1 and tadD were essential for the biosynthesis of Flp pilus and that the pilus played important roles during infection of A. pleuropneumoniae.

Identifiants

DOI: 10.1016/j.micpath.2018.11.010 PMID: 30419341
pubmed: 30419341
pii: S0882-4010(18)31209-9
doi: 10.1016/j.micpath.2018.11.010
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Fimbriae Proteins 147680-16-8

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

310-317

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Tingting Li (T)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Hubei Animal Disease Prevention and Control Center, Wuhan, Hubei, 430070, China.

Qiuhong Zhang (Q)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.

Rong Wang (R)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.

Sihua Zhang (S)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Jie Pei (J)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Hubei Animal Disease Prevention and Control Center, Wuhan, Hubei, 430070, China.

Yaokun Li (Y)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Lu Li (L)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Wuhan, Hubei, 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei, 430070, China. Electronic address: lilu@mail.hzau.edu.cn.

Rui Zhou (R)

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Wuhan, Hubei, 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei, 430070, China. Electronic address: rzhou@mail.hzau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Pasteurellaceae › Infections à Actinobacillus › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pasteurellaceae › Actinobacillus › Actinobacillus pleuropneumoniae › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Eucaryotes › Animaux › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques bactériens › Adhérence bactérienne › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes microbiologiques › Biofilms › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de la membrane externe bactérienne › Protéines de fimbriae › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Structures bactériennes › Fimbriae bactériens › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Gènes bactériens › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Recombinaison homologue › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée BALB C › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes microbiologiques › Viabilité microbienne › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes du système immunitaire › Phagocytose › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Délétion de séquence › The roles of flp1 and tadD in Actinobacillus...
questionsmedicales.fr › Phénomènes microbiologiques › Virulence › The roles of flp1 and tadD in Actinobacillus...