Interaction of the mycotoxin metabolite dihydrocitrinone with serum albumin.
Albumin-ligand interaction
Citrinin
Dihydrocitrinone
Fluorescence spectroscopy
Serum albumin
Journal
Mycotoxin research
ISSN: 1867-1632
Titre abrégé: Mycotoxin Res
Pays: Germany
ID NLM: 8807334
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
26
07
2018
accepted:
06
11
2018
revised:
04
11
2018
pubmed:
15
11
2018
medline:
14
6
2019
entrez:
15
11
2018
Statut:
ppublish
Résumé
Citrinin (CIT) is a nephrotoxic mycotoxin produced by Penicillium, Monascus, and Aspergillus species. CIT appears as a contaminant in cereals, cereal-based products, fruits, nuts, and spices. During the biotransformation of CIT, its major urinary metabolite dihydrocitrinone (DHC) is formed. Albumin interacts with several compounds (including mycotoxins) affecting their tissue distribution and elimination. CIT-albumin interaction is known; however, the complex formation of DHC with albumin has not been reported previously. In this study, we aimed to investigate the interaction of DHC with albumin, employing fluorescence spectroscopy, circular dichroism, and molecular modeling studies. Furthermore, species differences and thermodynamics of the interaction as well as the effects of albumin on the acute in vitro toxicity of DHC and CIT were also tested. Our main observations/conclusions are as follows: (1) Fluorescence signal of DHC is strongly enhanced by albumin. (2) Formation of DHC-albumin complexes is supported by both fluorescence spectroscopic and circular dichroism studies. (3) DHC forms similarly stable complexes with human albumin (K~10
Identifiants
pubmed: 30426325
doi: 10.1007/s12550-018-0336-z
pii: 10.1007/s12550-018-0336-z
doi:
Substances chimiques
Mycotoxins
0
Poisons
0
Serum Albumin
0
Citrinin
3S697X6SNZ
dihydrocitrinone
65718-85-6
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
129-139Subventions
Organisme : Hungarian National Research, Development and Innovation Office
ID : FK125166
Organisme : Hungarian National Research, Development and Innovation Office
ID : K123836
Organisme : Deutsche Forschungsgemeinschaft
ID : GRK1143
Références
Protein Sci. 2002 Jul;11(7):1729-37
pubmed: 12070326
Clin Microbiol Rev. 2003 Jul;16(3):497-516
pubmed: 12857779
FEBS Lett. 2006 Feb 20;580(5):1447-50
pubmed: 16460734
Wien Klin Wochenschr. 2008;120(13-14):402-7
pubmed: 18726664
J Comput Chem. 2009 Dec;30(16):2785-91
pubmed: 19399780
Arh Hig Rada Toksikol. 2009 Dec;60(4):457-64
pubmed: 20061247
Proteins. 2010 Jun;78(8):1950-8
pubmed: 20408171
Protein Sci. 2011 May;20(5):880-93
pubmed: 21413095
Proteins. 2012 Feb;80(2):374-81
pubmed: 22095872
Mol Aspects Med. 2012 Jun;33(3):209-90
pubmed: 22230555
Mol Pharm. 2013 May 6;10(5):1668-82
pubmed: 23473402
Biochim Biophys Acta. 2013 Dec;1830(12):5435-43
pubmed: 23665585
Arch Toxicol. 2014 May;88(5):1097-107
pubmed: 24577378
Arch Toxicol. 2015 Nov;89(11):1993-2005
pubmed: 25209565
Arch Toxicol. 2015 Apr;89(4):573-8
pubmed: 25224402
J Pharm Biomed Anal. 2015 Mar 25;107:325-32
pubmed: 25637820
Mycotoxin Res. 2015 Aug;31(3):127-36
pubmed: 25957672
Toxins (Basel). 2015 Dec 01;7(12):5155-66
pubmed: 26633504
J Pharmacol Toxicol Methods. 2016 May-Jun;79:45-54
pubmed: 26777595
J Pharm Biomed Anal. 2017 Feb 20;135:106-115
pubmed: 28012306
Food Chem Toxicol. 2017 Dec;110:130-141
pubmed: 28993214
Toxicol Lett. 2018 Jan 5;282:43-48
pubmed: 29030270
Mycotoxin Res. 2018 Mar;34(1):59-67
pubmed: 29143924
Mycotoxin Res. 2018 May;34(2):141-150
pubmed: 29442217
Toxins (Basel). 2018 Jun 22;10(7):
pubmed: 29932113
Mycotoxin Res. 2018 Nov;34(4):269-278
pubmed: 30014206
J Toxicol Environ Health. 1983 Aug-Sep;12(2-3):283-9
pubmed: 6655736
Anal Biochem. 1981 Jan 15;110(2):295-307
pubmed: 7235216
Biochemistry. 1981 May 26;20(11):3096-102
pubmed: 7248271
Experientia. 1977 May 15;33(5):598-9
pubmed: 862781