Chemometric analysis of porcine, bovine and ovine heparins.
Animals
Anticoagulants
/ analysis
Binding Sites
Cattle
Data Mining
Drug Contamination
/ prevention & control
Heparin
/ analysis
Intestinal Mucosa
/ chemistry
Oligosaccharides
/ chemistry
Principal Component Analysis
Proton Magnetic Resonance Spectroscopy
/ instrumentation
Quality Control
Sheep
Software
Swine
(1)H NMR spectroscopy
Animal origin
Chemometrics
Classification
Heparin
Mass spectrometry
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
05 Feb 2019
05 Feb 2019
Historique:
received:
12
10
2018
accepted:
29
10
2018
pubmed:
15
11
2018
medline:
20
3
2019
entrez:
15
11
2018
Statut:
ppublish
Résumé
Heparin is a polysaccharide anticoagulant drug isolated from animal tissues. There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption. The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins. These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site. Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
Identifiants
pubmed: 30428408
pii: S0731-7085(18)32315-X
doi: 10.1016/j.jpba.2018.10.052
pii:
doi:
Substances chimiques
Anticoagulants
0
Oligosaccharides
0
Heparin
9005-49-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
345-352Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.