Isolation, structural characterization, and immunostimulatory activity of a new water-soluble polysaccharide and its sulfated derivative from Citrus medica L. var. sarcodactylis.
Antioxidant activity
Citrus medica L. var. sarcodactylis
Immunomodulatory activity
Polysaccharide
Structure determination
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Feb 2019
15 Feb 2019
Historique:
received:
09
08
2018
revised:
03
10
2018
accepted:
12
11
2018
pubmed:
18
11
2018
medline:
11
4
2019
entrez:
17
11
2018
Statut:
ppublish
Résumé
The homogeneous heteropolysaccharide CMSPW90-1 was first purified from bergamot by DEAE Sepharose Fast Flow and Sephadex G-75 column with a molecular weight of 18.8 kDa. The structure of CMSPW90-1 was elucidated with high-performance gel permeation chromatography, gas chromatography-mass spectrometry, infrared spectrum, methylation, nuclear magnetic resonance (NMR), Congo red test, and circular dichroism. In comparison with CMSPW90-1, the sulfated derivative CMSPW90-M1 showed significant ultra-structural differences with a molecular weight of 75.4 kDa. The antioxidant and immunomodulatory activities of CMSPW90-M1 were examined to determine the relationships of structure-bioactivity. CMSPW90-M1 exhibited stronger scavenging activities for DPPH and ABTS
Identifiants
pubmed: 30445084
pii: S0141-8130(18)34155-2
doi: 10.1016/j.ijbiomac.2018.11.113
pii:
doi:
Substances chimiques
Adjuvants, Immunologic
0
Polysaccharides
0
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
500-511Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.