Ectonucleoside Triphosphate Diphosphohydrolase-3 Antibody Targets Adult Human Pancreatic β Cells for In Vitro and In Vivo Analysis.
Adenosine Triphosphatases
/ metabolism
Adult
Animals
Biomarkers
/ metabolism
Cells, Cultured
Diabetes Mellitus, Type 1
/ metabolism
Diabetes Mellitus, Type 2
/ metabolism
Humans
Insulin-Secreting Cells
/ metabolism
Islets of Langerhans
/ metabolism
Male
Membrane Proteins
/ metabolism
Mice
Mice, Inbred NOD
Pancreas
/ metabolism
Young Adult
development
diabetes
glucagon
imaging
insulin
islet
pancreas
Journal
Cell metabolism
ISSN: 1932-7420
Titre abrégé: Cell Metab
Pays: United States
ID NLM: 101233170
Informations de publication
Date de publication:
05 03 2019
05 03 2019
Historique:
received:
30
05
2018
revised:
15
08
2018
accepted:
19
10
2018
pmc-release:
05
03
2020
pubmed:
20
11
2018
medline:
12
5
2020
entrez:
20
11
2018
Statut:
ppublish
Résumé
Identification of cell-surface markers specific to human pancreatic β cells would allow in vivo analysis and imaging. Here we introduce a biomarker, ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase3), that is expressed on the cell surface of essentially all adult human β cells, including those from individuals with type 1 or type 2 diabetes. NTPDase3 is expressed dynamically during postnatal human pancreas development, appearing first in acinar cells at birth, but several months later its expression declines in acinar cells while concurrently emerging in islet β cells. Given its specificity and membrane localization, we utilized an NTPDase3 antibody for purification of live human β cells as confirmed by transcriptional profiling, and, in addition, for in vivo imaging of transplanted human β cells. Thus, NTPDase3 is a cell-surface biomarker of adult human β cells, and the antibody directed to this protein should be a useful new reagent for β cell sorting, in vivo imaging, and targeting.
Identifiants
pubmed: 30449685
pii: S1550-4131(18)30639-9
doi: 10.1016/j.cmet.2018.10.007
pmc: PMC6402969
mid: NIHMS1510722
pii:
doi:
Substances chimiques
Biomarkers
0
Membrane Proteins
0
Adenosine Triphosphatases
EC 3.6.1.-
ectoATPase
EC 3.6.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
745-754.e4Subventions
Organisme : NIDDK NIH HHS
ID : UC4 DK104218
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK104211
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK058404
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK072473
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK112232
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK089572
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY008126
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA068485
Pays : United States
Organisme : NIDDK NIH HHS
ID : F30 DK118830
Pays : United States
Organisme : NIH HHS
ID : U42 OD011158
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK098085
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK108120
Pays : United States
Organisme : NIH HHS
ID : S10 OD021630
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK098085
Pays : United States
Organisme : NIDDK NIH HHS
ID : U2C DK059637
Pays : United States
Organisme : BLRD VA
ID : I01 BX000666
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK094199
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK020593
Pays : United States
Organisme : NIDDK NIH HHS
ID : R24 DK106755
Pays : United States
Organisme : NIDDK NIH HHS
ID : K01 DK117969
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK104218
Pays : United States
Organisme : NIH HHS
ID : S10 OD016245
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK097829
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK059637
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK123716
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.
Références
Nat Rev Endocrinol. 2015 Apr;11(4):228-41
pubmed: 25687993
Diabetologia. 2008 Jan;51(1):91-100
pubmed: 17994216
Cell Tissue Res. 2001 Dec;306(3):373-83
pubmed: 11735037
Pancreas. 2008 Apr;36(3):279-83
pubmed: 18362842
Nat Med. 2008 May;14(5):574-8
pubmed: 18327249
Bioinformatics. 2009 May 1;25(9):1105-11
pubmed: 19289445
J Clin Invest. 2013 Mar;123(3):1275-84
pubmed: 23434589
Diabetologia. 2018 Dec;61(12):2512-2515
pubmed: 29955934
Trends Mol Med. 2013 Jun;19(6):355-67
pubmed: 23601906
FEBS J. 2009 Jan;276(2):479-96
pubmed: 19120451
Neuroscience. 2006;137(4):1331-46
pubmed: 16338080
Nat Commun. 2016 Jul 11;7:11756
pubmed: 27399229
Semin Nucl Med. 2010 May;40(3):167-81
pubmed: 20350626
J Clin Invest. 2019 Jan 2;129(1):246-251
pubmed: 30507613
Diabetes Obes Metab. 2016 Jan;18(1):6-15
pubmed: 26228188
Purinergic Signal. 2012 Sep;8(3):437-502
pubmed: 22555564
Diabetologia. 2016 Jul;59(7):1340-1349
pubmed: 27094935
Adv Pharmacol. 2011;61:301-32
pubmed: 21586363
Purinergic Signal. 2006 Jun;2(2):409-30
pubmed: 18404480
Am J Physiol Endocrinol Metab. 2016 Nov 1;311(5):E859-E868
pubmed: 27624103
Stem Cell Res. 2008 Sep;1(3):183-94
pubmed: 19383399
Am J Physiol Endocrinol Metab. 2010 Oct;299(4):E647-56
pubmed: 20682839
Eur J Pharm Sci. 2009 May 12;37(2):67-75
pubmed: 19429412
Am J Physiol Endocrinol Metab. 2013 Nov 15;305(10):E1319-26
pubmed: 24085034
Diabetes. 2015 Sep;64(9):3172-81
pubmed: 25931473
Diabetologia. 2011 Nov;54(11):2832-44
pubmed: 21882062
Am J Transplant. 2003 Sep;3(9):1135-42
pubmed: 12919094
Diabetes. 2004 May;53(5):1318-25
pubmed: 15111502
Biochem Biophys Res Commun. 2004 Feb 20;314(4):976-83
pubmed: 14751228
BMC Biol. 2011 May 31;9:34
pubmed: 21627854
Diabetes. 2018 Oct;67(10):2012-2018
pubmed: 30045920
Genome Biol. 2010;11(3):R25
pubmed: 20196867
Cell Metab. 2014 Mar 4;19(3):498-511
pubmed: 24561261
Diabetologia. 2014 Dec;57(12):2535-45
pubmed: 25208758
J Vis Exp. 2013 Mar 10;(73):e50466
pubmed: 23524511
Am J Physiol Endocrinol Metab. 2015 Apr 1;308(7):E592-602
pubmed: 25648831
FASEB J. 2013 Apr;27(4):1610-20
pubmed: 23299857
Cell Rep. 2018 Mar 6;22(10):2667-2676
pubmed: 29514095
Mol Cell Endocrinol. 2003 Jun 30;204(1-2):141-54
pubmed: 12850289
Cell Metab. 2016 Oct 11;24(4):593-607
pubmed: 27667667
Nat Protoc. 2008;3(8):1278-86
pubmed: 18714296
Brief Bioinform. 2013 Nov;14(6):671-83
pubmed: 22988256
Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20719-24
pubmed: 22143775
Mol Cell Endocrinol. 2011 Jun 6;339(1-2):144-50
pubmed: 21539888