Effect of CTLA4-Ig (abatacept) treatment on T cells and B cells in peripheral blood of patients with polymyositis and dermatomyositis.
CyTOF/ mass cytometry
T cell phenotype
abatacept
dermatomyositis
polymyositis
Journal
Scandinavian journal of immunology
ISSN: 1365-3083
Titre abrégé: Scand J Immunol
Pays: England
ID NLM: 0323767
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
12
06
2018
revised:
07
11
2018
accepted:
08
11
2018
pubmed:
20
11
2018
medline:
8
1
2019
entrez:
20
11
2018
Statut:
ppublish
Résumé
We aimed to evaluate in vivo effects of abatacept on phenotypes of T and B cells in the circulation of myositis patients in a sub-study of the ARTEMIS trial. Twelve patients with paired frozen PBMCs before and after 6-month abatacept treatment were included in this sub-study where mass cytometry (CyTOF) was chosen as a technology to be tested for its utility in a real-life clinical immune monitoring setting. Using CyTOF, the peripheral T cell phenotypes demonstrated considerable variation over time and between individuals precluding the identification of treatment-specific changes. We therefore conclude that studies of patient cohorts displaying wide clinical heterogeneity using mass cytometry must be relatively large in order to be suited for discovery research and immune monitoring. Still, we did find some correlations with functional muscle outcome, namely positive correlations between the ratio of CD4
Substances chimiques
Immunosuppressive Agents
0
Abatacept
7D0YB67S97
Types de publication
Journal Article
Randomized Controlled Trial
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12732Subventions
Organisme : China Scholarship Council
Organisme : Ministry of Health in the Czech Republic for Conceptual Development of Research Organization
ID : 00023728
Organisme : Bristol-Myers Squibb International Corporation
Organisme : Swedish Rheumatism Association
Organisme : Swedish Research Council
ID : K2014-52X-14045-14-3
Organisme : King Gustaf V 80 Year Foundation
Organisme : regional agreement on medical training and clinical research (ALF) between Stockholm County Council and Karolinska Institutet
Informations de copyright
© 2018 The Foundation for the Scandinavian Journal of Immunology.