The Host DHX9 DExH-Box Helicase Is Recruited to Chikungunya Virus Replication Complexes for Optimal Genomic RNA Translation.
Animals
Cell Line
Cell Membrane
/ metabolism
Chikungunya Fever
/ virology
Chikungunya virus
/ metabolism
Chlorocebus aethiops
DEAD-box RNA Helicases
/ genetics
DNA Helicases
/ metabolism
Genomics
HEK293 Cells
HeLa Cells
Humans
Neoplasm Proteins
/ genetics
Protein Biosynthesis
/ genetics
RNA Helicases
/ metabolism
RNA, Viral
/ metabolism
Vero Cells
Virus Replication
/ genetics
DHX9
RNA helicase
chikungunya virus
nsP3
viral replication
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
15 02 2019
15 02 2019
Historique:
received:
11
10
2018
accepted:
19
11
2018
pubmed:
23
11
2018
medline:
18
12
2019
entrez:
23
11
2018
Statut:
epublish
Résumé
Beyond their role in cellular RNA metabolism, DExD/H-box RNA helicases are hijacked by various RNA viruses in order to assist replication of the viral genome. Here, we identify the DExH-box RNA helicase 9 (DHX9) as a binding partner of chikungunya virus (CHIKV) nsP3 mainly interacting with the C-terminal hypervariable domain. We show that during early CHIKV infection, DHX9 is recruited to the plasma membrane, where it associates with replication complexes. At a later stage of infection, DHX9 is, however, degraded through a proteasome-dependent mechanism. Using silencing experiments, we demonstrate that while DHX9 negatively controls viral RNA synthesis, it is also required for optimal mature nonstructural protein translation. Altogether, this study identifies DHX9 as a novel cofactor for CHIKV replication in human cells that differently regulates the various steps of CHIKV life cycle and may therefore mediate a switch in RNA usage from translation to replication during the earliest steps of CHIKV replication.
Identifiants
pubmed: 30463980
pii: JVI.01764-18
doi: 10.1128/JVI.01764-18
pmc: PMC6364007
pii:
doi:
Substances chimiques
Neoplasm Proteins
0
RNA, Viral
0
DHX9 protein, human
EC 3.6.1.-
DNA Helicases
EC 3.6.4.-
DEAD-box RNA Helicases
EC 3.6.4.13
RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2019 American Society for Microbiology.
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