Inhibition of alpha-hemolysin expression by resveratrol attenuates Staphylococcus aureus virulence.
A549 Cells
Animals
Bacterial Toxins
/ antagonists & inhibitors
Coculture Techniques
Disease Models, Animal
Enzyme Inhibitors
/ metabolism
Hemolysin Proteins
/ antagonists & inhibitors
Humans
Mice
Pneumonia, Staphylococcal
/ drug therapy
Resveratrol
/ metabolism
Staphylococcus aureus
/ drug effects
Treatment Outcome
Virulence
/ drug effects
Alpha-hemolysin
Anti-infective agent
Resveratrol
Staphylococcus aureus
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
28
04
2018
revised:
19
11
2018
accepted:
19
11
2018
pubmed:
24
11
2018
medline:
6
3
2019
entrez:
24
11
2018
Statut:
ppublish
Résumé
Staphylococcus aureus is an important zoonotic pathogen that causes a variety of life-threatening diseases. The increasing emergence of drug resistance further complicates the treatment of S. aureus infections. The critical role of alpha-hemolysin (Hla) in virulence renders this toxin an ideal target for the development of anti-infective agents for S. aureus. Here, We found that resveratrol, a natural compound widely found in fruits without antibacterial activity, could effectively inhibit Hla expression via down-regulating the transcription of hla, the gene that encodes Hla, and RNAIII, the effector molecule of the agr system. The addition of resveratrol to a co-culture system of S. aureus and A549 cells significantly alleviated bacteria-mediated cellular injury. Furthermore, treatment with resveratrol effectively protected mice from S. aureus pneumonia. Our results established resveratrol as an effective Hla inhibitor that reduces Hla expression without antimicrobial activity and can be further developed into novel therapeutics against S. aureus infections.
Identifiants
pubmed: 30468853
pii: S0882-4010(18)30753-8
doi: 10.1016/j.micpath.2018.11.027
pii:
doi:
Substances chimiques
Bacterial Toxins
0
Enzyme Inhibitors
0
Hemolysin Proteins
0
staphylococcal alpha-toxin
0
Resveratrol
Q369O8926L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
85-90Informations de copyright
Copyright © 2018. Published by Elsevier Ltd.