Berberine chloride mediates its antileishmanial activity by inhibiting Leishmania mitochondria.
Adenosine Triphosphate
/ metabolism
Antiprotozoal Agents
/ pharmacology
Apoptosis
/ drug effects
Berberine Alkaloids
/ pharmacology
Leishmania
/ drug effects
Membrane Potential, Mitochondrial
/ drug effects
Mitochondria
/ drug effects
Oxidation-Reduction
/ drug effects
Oxidative Stress
/ drug effects
Reactive Oxygen Species
/ metabolism
Superoxides
/ metabolism
Berberine chloride
Leishmania
Mitochondria
Mitochondrial superoxide
Promastigotes
Reactive oxygen species (ROS)
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
06
08
2018
accepted:
14
11
2018
pubmed:
25
11
2018
medline:
22
3
2019
entrez:
25
11
2018
Statut:
ppublish
Résumé
Berberine chloride, a plant-derived isoquinoline alkaloid, has been demonstrated to have leishmanicidal activity, which is mediated by generation of a redox imbalance and depolarization of the mitochondrial membrane, resulting in a caspase-independent apoptotic-like cell death. However, its impact on mitochondrial function remains to be delineated and is the focus of this study. In UR6 promastigotes, berberine chloride demonstrated a dose-dependent increase in generation of reactive oxygen species and mitochondrial superoxide, depolarization of the mitochondrial membrane potential, a dose-dependent inhibition of mitochondrial complexes I-III and II-III, along with a substantial depletion of ATP, collectively suggesting inhibition of parasite mitochondria. Accordingly, the oxidative stress induced by berberine chloride resulting in an apoptotic-like cell death in Leishmania can be exploited as a potent chemotherapeutic strategy, mitochondria being a prime contributor.
Identifiants
pubmed: 30470927
doi: 10.1007/s00436-018-6157-3
pii: 10.1007/s00436-018-6157-3
doi:
Substances chimiques
Antiprotozoal Agents
0
Berberine Alkaloids
0
Reactive Oxygen Species
0
Superoxides
11062-77-4
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
335-345Subventions
Organisme : International Bilateral Cooperation Division, Dept of Science & Technology (DST), Govt. of India
ID : INT/AUSTRIA/BMWF/P-06/2017
Organisme : Fund for Improvement of S&T infrastructure in Universities and Higher Educational Institutions (FIST) Program, DST, Govt. of India
ID : SR/FST/LS1-049/2010
Organisme : Dept. of Health Research, Govt. of India, 'Establishment of Multidisciplinary Research Unit'
ID : V.25011/103/2016-HR
Organisme : Austrian Science Fund
ID : P 27814-B22
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