Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.

Amino Acids / chemistry Chemistry Techniques, Analytical / instrumentation Chromatography, Supercritical Fluid Polysaccharides / chemistry Stereoisomerism
Extra column band-broadening Mobile phase composition Native amino-acids Polysaccharide-based chiral stationary phases Separation of enantiomers

Journal

Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488

Informations de publication

Date de publication:
25 Jan 2019
Historique:
received: 02 10 2018
revised: 14 11 2018
accepted: 20 11 2018
pubmed: 1 12 2018
medline: 6 3 2019
entrez: 1 12 2018
Statut: ppublish

Résumé

In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC). Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors. Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids. In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.

Identifiants

DOI: 10.1016/j.chroma.2018.11.049 PMID: 30497824
pubmed: 30497824
pii: S0021-9673(18)31449-3
doi: 10.1016/j.chroma.2018.11.049
pii:
doi:

Substances chimiques

Amino Acids 0
Polysaccharides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

207-212

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Emmanuelle Lipka (E)

Unité 995 INSERM, Université de Lille, Lille Inflammation Research International Center, Laboratoire de Chimie Analytique-Faculté de Pharmacie de Lille, BP 83 - 59006 Lille, France. Electronic address: emmanuelle.lipka@univ-lille.fr.

Anca-Elena Dascalu (AE)

Unité 995 INSERM, Université de Lille, Lille Inflammation Research International Center, Laboratoire de Chimie Analytique-Faculté de Pharmacie de Lille, BP 83 - 59006 Lille, France; Hautes Etudes d'Ingénieur (HEI), Yncréa Hauts-de-France, UCLille, Laboratoire de Pharmacochimie, 13 rue de Toul, F-59046 Lille, France; 'Alexandru Ioan Cuza' University of Iasi, Faculty of Chemistry, Bd. Carol I nr. 11, 700506 Iasi, Romania.

Yasmine Messara (Y)

Unité 995 INSERM, Université de Lille, Lille Inflammation Research International Center, Laboratoire de Chimie Analytique-Faculté de Pharmacie de Lille, BP 83 - 59006 Lille, France.

Eka Tsutsqiridze (E)

Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.

Tivadar Farkas (T)

Phenomenex Inc., 411 Madrid Ave., Torrance, 90501 CA, USA.

Bezhan Chankvetadze (B)

Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia. Electronic address: jpba_bezhan@yahoo.com.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Separation of enantiomers of native amino...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Separation of enantiomers of native amino...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase supercritique › Separation of enantiomers of native amino...
questionsmedicales.fr › Glucides › Polyosides › Separation of enantiomers of native amino...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes de chimie organique › Isomérie › Stéréoisomérie › Separation of enantiomers of native amino...