A versatile remote control system for functional expression of bacterial virulence genes based on the tetA promoter.
Adhesins, Bacterial
/ genetics
Antiporters
/ genetics
Bacterial Proteins
/ genetics
Enteropathogenic Escherichia coli
/ genetics
Flagella
/ genetics
Gene Expression Regulation, Bacterial
/ drug effects
HeLa Cells
Humans
Operon
/ drug effects
Promoter Regions, Genetic
/ drug effects
Salmonella enterica
/ genetics
Tetracycline Resistance
/ drug effects
Tetracyclines
/ pharmacology
Virulence
/ drug effects
Yersinia enterocolitica
/ genetics
Autotransporter adhesins
Fimbrial adhesins
Heterologous expression system
Tet repressor
Type III secretion system
Journal
International journal of medical microbiology : IJMM
ISSN: 1618-0607
Titre abrégé: Int J Med Microbiol
Pays: Germany
ID NLM: 100898849
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
26
07
2018
revised:
21
10
2018
accepted:
14
11
2018
pubmed:
7
12
2018
medline:
6
7
2019
entrez:
4
12
2018
Statut:
ppublish
Résumé
The expression of bacterial virulence factors is controlled in response to host or environmental factors and most virulence genes are not expressed under laboratory conditions. Investigations of molecular structures and cellular functions of bacterial virulence factors demand systems for experimentally controlled expression. We describe a simple and robust system that is based on the tetA promoter and the cognate repressor TetR. Expression under control of P
Identifiants
pubmed: 30501934
pii: S1438-4221(18)30396-5
doi: 10.1016/j.ijmm.2018.11.001
pii:
doi:
Substances chimiques
Adhesins, Bacterial
0
Antiporters
0
Bacterial Proteins
0
Tetracyclines
0
tetA protein, Bacteria
0
4-epianhydrotetracycline
680VDL31MX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
54-65Informations de copyright
Copyright © 2018 Elsevier GmbH. All rights reserved.