Comparison of the performance of the IDEXX SediVue Dx® with manual microscopy for the detection of cells and 2 crystal types in canine and feline urine.
automated analyzer
cat
dog
urinalysis
urine formed elements
urine sediment
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
26
10
2017
accepted:
05
10
2018
pubmed:
5
12
2018
medline:
8
2
2019
entrez:
5
12
2018
Statut:
ppublish
Résumé
Microscopic evaluation of urine is inconsistently performed in veterinary clinics. The IDEXX SediVue Dx® Urine Sediment Analyzer (SediVue) recently was introduced for automated analysis of canine and feline urine and may facilitate performance of urinalyses in practice. Compare the performance of the SediVue with manual microscopy for detecting clinically relevant numbers of cells and 2 crystal types. Five-hundred thirty urine samples (82% canine, 18% feline). For SediVue analysis (software versions [SW] 1.0.0.0 and 1.0.1.3), uncentrifuged urine was pipetted into a cartridge. Images were captured and processed using a convolutional neural network algorithm. For manual microscopy, urine was centrifuged to obtain sediment. To determine sensitivity and specificity of the SediVue compared with manual microscopy, thresholds were set at ≥5/high power field (hpf) for red blood cells (RBC) and white blood cells (WBC) and ≥1/hpf for squamous epithelial cells (sqEPI), non-squamous epithelial cells (nsEPI), struvite crystals (STR), and calcium oxalate dihydrate crystals (CaOx Di). The sensitivity of the SediVue (SW1.0.1.3) was 85%-90% for the detection of RBC, WBC, and STR; 75% for CaOx Di; 71% for nsEPI; and 33% for sqEPI. Specificity was 99% for sqEPI and CaOx Di; 87%-90% for RBC, WBC, and nsEPI; and 84% for STR. Compared to SW1.0.0.0, SW1.0.1.3 had increased sensitivity but decreased specificity. Performance was similar for canine versus feline and fresh versus stored urine samples. The SediVue exhibits good agreement with manual microscopy for the detection of most formed elements evaluated, but improvement is needed for epithelial cells.
Sections du résumé
BACKGROUND
BACKGROUND
Microscopic evaluation of urine is inconsistently performed in veterinary clinics. The IDEXX SediVue Dx® Urine Sediment Analyzer (SediVue) recently was introduced for automated analysis of canine and feline urine and may facilitate performance of urinalyses in practice.
OBJECTIVE
OBJECTIVE
Compare the performance of the SediVue with manual microscopy for detecting clinically relevant numbers of cells and 2 crystal types.
SAMPLES
METHODS
Five-hundred thirty urine samples (82% canine, 18% feline).
METHODS
METHODS
For SediVue analysis (software versions [SW] 1.0.0.0 and 1.0.1.3), uncentrifuged urine was pipetted into a cartridge. Images were captured and processed using a convolutional neural network algorithm. For manual microscopy, urine was centrifuged to obtain sediment. To determine sensitivity and specificity of the SediVue compared with manual microscopy, thresholds were set at ≥5/high power field (hpf) for red blood cells (RBC) and white blood cells (WBC) and ≥1/hpf for squamous epithelial cells (sqEPI), non-squamous epithelial cells (nsEPI), struvite crystals (STR), and calcium oxalate dihydrate crystals (CaOx Di).
RESULTS
RESULTS
The sensitivity of the SediVue (SW1.0.1.3) was 85%-90% for the detection of RBC, WBC, and STR; 75% for CaOx Di; 71% for nsEPI; and 33% for sqEPI. Specificity was 99% for sqEPI and CaOx Di; 87%-90% for RBC, WBC, and nsEPI; and 84% for STR. Compared to SW1.0.0.0, SW1.0.1.3 had increased sensitivity but decreased specificity. Performance was similar for canine versus feline and fresh versus stored urine samples.
CONCLUSIONS AND CLINICAL IMPORTANCE
CONCLUSIONS
The SediVue exhibits good agreement with manual microscopy for the detection of most formed elements evaluated, but improvement is needed for epithelial cells.
Identifiants
pubmed: 30511380
doi: 10.1111/jvim.15341
pmc: PMC6335506
doi:
Substances chimiques
Calcium Oxalate
2612HC57YE
Struvite
AW3EJL1462
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
167-177Subventions
Organisme : IDEXX Laboratories, Inc
Informations de copyright
© 2018 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
Références
Clin Chem Lab Med. 1998 Dec;36(12):909-17
pubmed: 9915222
Biotechnol Appl Biochem. 2009 Jun;53(Pt 2):139-44
pubmed: 19021504
Clin Chim Acta. 2007 Sep;384(1-2):28-34
pubmed: 17604012
Clin Lab. 2014;60(4):693-7
pubmed: 24779308
Clin Chem. 1974 Apr;20(4):436-9
pubmed: 4818195
J Clin Lab Anal. 2008;22(4):262-70
pubmed: 18623125
Acta Clin Belg. 2017 Apr;72(2):91-94
pubmed: 27796180
Clin Lab Med. 1988 Sep;8(3):449-61
pubmed: 3168417
Clin Chim Acta. 2006 Oct;372(1-2):54-64
pubmed: 16696963
Clin Chem Lab Med. 2005;43(9):967-70
pubmed: 16176179
Am J Clin Pathol. 2001 Dec;116(6):872-8
pubmed: 11764076
J Vet Intern Med. 2019 Jan;33(1):167-177
pubmed: 30511380
Clin Chem Lab Med. 1999 Jul;37(7):753-64
pubmed: 10510734
Clin Chem Lab Med. 2007;45(9):1251-6
pubmed: 17635081
J Clin Lab Anal. 2013 Jul;27(4):312-6
pubmed: 23852791
Clin J Am Soc Nephrol. 2009 Mar;4(3):567-71
pubmed: 19261816
Clin Chim Acta. 2010 Feb;411(3-4):147-54
pubmed: 19861122
Biometrics. 1977 Mar;33(1):159-74
pubmed: 843571