Amphoteric poly(amido amine)s with adjustable balance between transfection efficiency and cytotoxicity for gene delivery.
Agmatine
/ chemistry
Animals
Cell Survival
/ drug effects
Chemistry Techniques, Synthetic
/ methods
Gene Transfer Techniques
Mice
Microscopy, Confocal
Models, Chemical
Molecular Structure
NIH 3T3 Cells
Polyamines
/ chemical synthesis
Polymers
/ chemical synthesis
Transfection
/ methods
gamma-Aminobutyric Acid
/ chemistry
Amphoteric polymers
Cytotoxicity
Gene delivery
Nuclear localization ability
Transfection efficiency
Journal
Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133
Informations de publication
Date de publication:
01 Mar 2019
01 Mar 2019
Historique:
received:
28
06
2018
revised:
24
10
2018
accepted:
19
11
2018
pubmed:
5
12
2018
medline:
4
6
2019
entrez:
5
12
2018
Statut:
ppublish
Résumé
In order to balance transfection efficiency and cytotoxicity as well as screen the optimal polymers for gene delivery, a series of amphoteric copolymers (poly(CBA-AGM/GABA)s) composed of different ratios between agmatine (AGM) and γ-aminobutyric acid (GABA) monomers were synthesized. The AGM containing positively charged guanidinium groups was used to improve transfection efficiency, while the GABA containing negatively charged carboxyl groups was used to decrease cytotoxicity. It is hypothesized that the amphoteric poly(CBA-AGM/GABA)s synthesized at the optimal ratio of both components would well balance transfection efficiency and cytotoxicity. By comparing these polymers' essential features in gene delivery, the ideal ratio between AGM and GABA was optimized. AGM80, which contained 80% AGM and 20% GABA, showed favorable properties for gene delivery, including moderate DNA condensation capacity, high cellular uptake, strong nuclear localization ability, high transfection efficiency, and low cytotoxicity, indicating that this polymer is very promising as a potent and nontoxic gene carrier.
Identifiants
pubmed: 30513469
pii: S0927-7765(18)30827-0
doi: 10.1016/j.colsurfb.2018.11.045
pii:
doi:
Substances chimiques
Poly(amidoamine)
0
Polyamines
0
Polymers
0
gamma-Aminobutyric Acid
56-12-2
Agmatine
70J407ZL5Q
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
10-17Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.