MODELLING γ-H2AX FOCI INDUCTION TO MIMIC LIMITATIONS IN THE SCORING TECHNIQUE.
Algorithms
Animals
DNA Breaks, Double-Stranded
/ radiation effects
Histones
/ radiation effects
Immunohistochemistry
Linear Energy Transfer
Mammary Neoplasms, Experimental
/ diagnostic imaging
Mice
Monte Carlo Method
Relative Biological Effectiveness
Software
Tumor Cells, Cultured
/ radiation effects
X-Rays
Journal
Radiation protection dosimetry
ISSN: 1742-3406
Titre abrégé: Radiat Prot Dosimetry
Pays: England
ID NLM: 8109958
Informations de publication
Date de publication:
01 May 2019
01 May 2019
Historique:
pubmed:
7
12
2018
medline:
5
6
2019
entrez:
7
12
2018
Statut:
ppublish
Résumé
An approach based on track-structure calculations has been developed to take account of artefacts occurring during γ-H2AX foci detection in 2D images of samples analyzed through immunocytochemistry. The need of this works stems from the observed saturation in foci yields measured after X-ray doses higher than few grays, hindering an unambiguous quantification of DNA damage and of radiation effectiveness. The proposed modelling approach allows to simulate the observer's point of view for foci scoring, mimicking the selection of a slice Δz of the cell nucleus due to the microscope depth of field, and applying a clustering algorithm to group together damages within a resolution parameter r. Calculation results were benchmarked with experimental measurements at an early time-point for mouse breast cancer cells, irradiated with X-ray doses in the range 0-5 Gy. The model is able to reproduce the saturation in experimental data.
Identifiants
pubmed: 30520984
pii: 5231891
doi: 10.1093/rpd/ncy217
pmc: PMC6525333
doi:
Substances chimiques
H2AX protein, mouse
0
Histones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
121-125Subventions
Organisme : NIBIB NIH HHS
ID : P41 EB002033
Pays : United States
Informations de copyright
© The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.
Références
Int J Radiat Biol. 2012 May;88(5):439-47
pubmed: 22280362
PLoS One. 2013 Nov 29;8(11):e79541
pubmed: 24312182
Sci Rep. 2017 Mar 27;7:45161
pubmed: 28345622
Sci Rep. 2016 Dec 06;6:38295
pubmed: 27922110
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Mutat Res. 2011 Jun 3;711(1-2):49-60
pubmed: 21216255
Radiat Oncol. 2015 Aug 04;10:163
pubmed: 26238507
Int J Mol Sci. 2017 Sep 15;18(9):
pubmed: 28914798
PLoS One. 2015 Jun 11;10(6):e0129416
pubmed: 26067661
Sci Rep. 2017 Jan 17;7:40616
pubmed: 28094292