Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
09 01 2019
09 01 2019
Historique:
pubmed:
12
12
2018
medline:
10
6
2020
entrez:
12
12
2018
Statut:
ppublish
Résumé
The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium Anabaena PCC7120 with opposite fluorescence behavior. In fact, while W76S/Y179F displays, with respect to the wild-type protein, a nearly 10-fold increase in red-light emission, the second is not emissive. Thus, the W76S/Y179F, L83Q pair offers an unprecedented opportunity for the investigation of fluorescence enhancement in microbial rhodopsins, which is pursued by combining transient absorption spectroscopy and multiconfigurational quantum chemistry. The results of such an investigation point to an isomerization-blocking electronic effect as the direct cause of instantaneous (subpicosecond) fluorescence enhancement.
Identifiants
pubmed: 30532962
doi: 10.1021/jacs.8b09311
pmc: PMC6475468
mid: NIHMS1010559
doi:
Substances chimiques
Mutant Proteins
0
Rhodopsins, Microbial
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
262-271Subventions
Organisme : NIGMS NIH HHS
ID : R15 GM126627
Pays : United States
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