Local Acceleration of Neurofilament Transport at Nodes of Ranvier.
Animals
Axonal Transport
/ physiology
Axons
/ metabolism
Cells, Cultured
Female
Green Fluorescent Proteins
Male
Mice
Mice, Transgenic
Mitochondria
/ metabolism
Models, Theoretical
Myelin Sheath
/ metabolism
Nerve Fibers, Myelinated
/ metabolism
Neurofilament Proteins
/ metabolism
Ranvier's Nodes
/ metabolism
Tibial Nerve
/ cytology
Schwann cell
axonal transport
myelin
myelination
neurofilament
node of Ranvier
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140
Informations de publication
Date de publication:
23 01 2019
23 01 2019
Historique:
received:
31
08
2018
revised:
24
10
2018
accepted:
26
10
2018
pubmed:
14
12
2018
medline:
18
12
2019
entrez:
14
12
2018
Statut:
ppublish
Résumé
Myelinated axons are constricted at nodes of Ranvier. These constrictions are important physiologically because they increase the speed of saltatory nerve conduction, but they also represent potential bottlenecks for the movement of axonally transported cargoes. One type of cargo are neurofilaments, which are abundant space-filling cytoskeletal polymers that function to increase axon caliber. Neurofilaments move bidirectionally along axons, alternating between rapid movements and prolonged pauses. Strikingly, axon constriction at nodes is accompanied by a reduction in neurofilament number that can be as much as 10-fold in the largest axons. To investigate how neurofilaments navigate these constrictions, we developed a transgenic mouse strain that expresses a photoactivatable fluorescent neurofilament protein in neurons. We used the pulse-escape fluorescence photoactivation technique to analyze neurofilament transport in mature myelinated axons of tibial nerves from male and female mice of this strain
Identifiants
pubmed: 30541916
pii: JNEUROSCI.2272-18.2018
doi: 10.1523/JNEUROSCI.2272-18.2018
pmc: PMC6343641
doi:
Substances chimiques
Neurofilament Proteins
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
663-677Subventions
Organisme : NINDS NIH HHS
ID : P30 NS045758
Pays : United States
Organisme : NINDS NIH HHS
ID : P30 NS104177
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS038526
Pays : United States
Organisme : NIH HHS
ID : S10 OD010383
Pays : United States
Informations de copyright
Copyright © 2019 the authors 0270-6474/19/390664-15$15.00/0.
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