Frequency of superantigen encoding genes of Staphylococcus aureus isolates collected from multiple sclerosis (MS) patients and nasal carriers.


Journal

Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191

Informations de publication

Date de publication:
Feb 2019
Historique:
received: 26 04 2018
revised: 05 12 2018
accepted: 06 12 2018
pubmed: 17 12 2018
medline: 15 3 2019
entrez: 17 12 2018
Statut: ppublish

Résumé

Bacterial superantigens are potent T cell activators that can have acute or chronic effects on the central nervous system. In this study, the role of enterotoxins, exfoliative toxins and toxic shock syndrome toxin of Staphylococcus aureus was investigated in MS patients and healthy nasal carriers. Three-hundred fifty nasal swabs were collected from healthy nasal carriers (n = 210) and MS (n = 140) patients. Staphylococcus aureus superantigens were detected by multiplex PCR. Antimicrobial susceptibility pattern was performed using disk diffusion method. The highest rates of nasal colonization were seen in MS patients (46.42%). The rates of nasal colonization in the healthcare workers were 30.95%. The most commonly detected superantigens were SEA (31.5%), SEB (17.7%) and ETA (16.9%). The Staphylococcus aureus isolates had the highest levels of resistance against erythromycin (57.7%), clindamycin (55.4%) and co-trimoxazole (43.1%). All isolates were susceptible to vancomycin, linezolid, and mupirocin. Our results revealed that the frequency of superantigen producing Staphylococcus aureus isolates is high in the MS patients. As well as these isolates are sensitive to mupirocin. Thus it is better to use of mupirocin for nasal decolonization of Staphylococcus aureus in the MS patients.

Sections du résumé

BACKGROUND BACKGROUND
Bacterial superantigens are potent T cell activators that can have acute or chronic effects on the central nervous system.
OBJECTIVES OBJECTIVE
In this study, the role of enterotoxins, exfoliative toxins and toxic shock syndrome toxin of Staphylococcus aureus was investigated in MS patients and healthy nasal carriers.
METHODS METHODS
Three-hundred fifty nasal swabs were collected from healthy nasal carriers (n = 210) and MS (n = 140) patients. Staphylococcus aureus superantigens were detected by multiplex PCR. Antimicrobial susceptibility pattern was performed using disk diffusion method.
RESULTS RESULTS
The highest rates of nasal colonization were seen in MS patients (46.42%). The rates of nasal colonization in the healthcare workers were 30.95%. The most commonly detected superantigens were SEA (31.5%), SEB (17.7%) and ETA (16.9%). The Staphylococcus aureus isolates had the highest levels of resistance against erythromycin (57.7%), clindamycin (55.4%) and co-trimoxazole (43.1%). All isolates were susceptible to vancomycin, linezolid, and mupirocin.
CONCLUSION CONCLUSIONS
Our results revealed that the frequency of superantigen producing Staphylococcus aureus isolates is high in the MS patients. As well as these isolates are sensitive to mupirocin. Thus it is better to use of mupirocin for nasal decolonization of Staphylococcus aureus in the MS patients.

Identifiants

pubmed: 30553909
pii: S0882-4010(18)30739-3
doi: 10.1016/j.micpath.2018.12.010
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
Enterotoxins 0
Exfoliatins 0
Superantigens 0
toxic shock syndrome toxin 2, Staphylococcus aureus 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

316-319

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Javid Sadeghi (J)

Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: sadeghij@tbzmed.ac.ir.

Naser Alizadeh (N)

Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: alizade.naser@ymail.com.

Mahin Ahangar Oskouei (M)

Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: ahangar1342@gmail.com.

Delara Laghusi (D)

Department of Social Medicine, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: dlaghusi@yahoo.com.

Daryush Savadi Oskouei (D)

Department of Neurology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: savadi_d@yahoo.com.

Masoud Nikanfar (M)

Department of Neurology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: masoudnikanfar@yahoo.com.

Mir Naser Seyyed Mousavi (MN)

Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: mns.mousavi90@yahoo.com.

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Classifications MeSH