Protective Effect of Selenium-L-methionine on Radiation-induced Acute Pneumonitis and Lung Fibrosis in Rat.
Animals
Dual Oxidases
/ metabolism
Interleukin-13
/ metabolism
Interleukin-13 Receptor alpha2 Subunit
/ metabolism
Interleukin-4
/ metabolism
Interleukin-4 Receptor alpha Subunit
/ metabolism
Male
Methionine
/ therapeutic use
Pneumonia
/ etiology
Pulmonary Fibrosis
/ complications
Radiation Injuries, Experimental
/ complications
Radiation-Protective Agents
/ therapeutic use
Rats
Rats, Wistar
Selenium
/ therapeutic use
IL-4. IL-13
Selenium-L-methionine
interleukin
lung
pneumonitis
radiation
radiotherapy.
Journal
Current clinical pharmacology
ISSN: 2212-3938
Titre abrégé: Curr Clin Pharmacol
Pays: United Arab Emirates
ID NLM: 101273158
Informations de publication
Date de publication:
2019
2019
Historique:
received:
05
09
2018
revised:
13
11
2018
accepted:
16
11
2018
pubmed:
18
12
2018
medline:
8
7
2020
entrez:
18
12
2018
Statut:
ppublish
Résumé
In this study, we aimed to detect the changes in the level of interleukin (IL)-4 and IL-13 cytokines and their downstream genes including interleukin-13 receptor subunit alpha-2 (IL13Ra2), interleukin-4 receptor subunit alpha-1 (IL4Ra1), dual oxidase 1 (DUOX1) and dual oxidase 2 (DUOX2). The protective effects of Selenium-L-methionine on radiation-induced histopathological damages and changes in the level of these cytokines and genes were detected. Four groups of 20 rats (5 rats in each) namely, control; Selenium-L-methionine, radiation and radiation plus Selenium-L-methionine were used in this study. 4 mg/kg of Selenium-Lmethionine was administered 1 day before irradiation and five consecutive days after irradiation. Irradiation was done using a dose of 15 Gy 60Co gamma rays at 109 cGy/min. All rats were sacrificed 10 weeks after irradiation for detecting changes in IL-4 and IL-13 cytokines, the expressions of IL13Ra2, IL4Ra1, Duox1 and Duox2 and histopathological changes. The level of IL-4 but not IL-13 increased after irradiation. This was associated with increased expression of IL4Ra1, Duox1 and Duox2, in addition to changes in morphological properties. Selenium-L-methionine could attenuate all injury markers following lung irradiation. Selenium-L-methionine can protect lung tissues against toxic effects of ionizing radiation. It is possible that the modulation of immune responses and redox interactions are involved in the radioprotective effect of this agent.
Sections du résumé
BACKGROUND
In this study, we aimed to detect the changes in the level of interleukin (IL)-4 and IL-13 cytokines and their downstream genes including interleukin-13 receptor subunit alpha-2 (IL13Ra2), interleukin-4 receptor subunit alpha-1 (IL4Ra1), dual oxidase 1 (DUOX1) and dual oxidase 2 (DUOX2). The protective effects of Selenium-L-methionine on radiation-induced histopathological damages and changes in the level of these cytokines and genes were detected.
METHODS
Four groups of 20 rats (5 rats in each) namely, control; Selenium-L-methionine, radiation and radiation plus Selenium-L-methionine were used in this study. 4 mg/kg of Selenium-Lmethionine was administered 1 day before irradiation and five consecutive days after irradiation. Irradiation was done using a dose of 15 Gy 60Co gamma rays at 109 cGy/min. All rats were sacrificed 10 weeks after irradiation for detecting changes in IL-4 and IL-13 cytokines, the expressions of IL13Ra2, IL4Ra1, Duox1 and Duox2 and histopathological changes.
RESULTS
The level of IL-4 but not IL-13 increased after irradiation. This was associated with increased expression of IL4Ra1, Duox1 and Duox2, in addition to changes in morphological properties. Selenium-L-methionine could attenuate all injury markers following lung irradiation.
CONCLUSION
Selenium-L-methionine can protect lung tissues against toxic effects of ionizing radiation. It is possible that the modulation of immune responses and redox interactions are involved in the radioprotective effect of this agent.
Identifiants
pubmed: 30556505
pii: CCP-EPUB-95234
doi: 10.2174/1574884714666181214101917
pmc: PMC7040518
doi:
Substances chimiques
Il4r protein, rat
0
Interleukin-13
0
Interleukin-13 Receptor alpha2 Subunit
0
Interleukin-4 Receptor alpha Subunit
0
Radiation-Protective Agents
0
Interleukin-4
207137-56-2
Methionine
AE28F7PNPL
Dual Oxidases
EC 1.11.1.-
Duox1 protein, rat
EC 1.6.3.1
Duox2 protein, rat
EC 1.6.3.1
Selenium
H6241UJ22B
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
157-164Informations de copyright
Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.
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