Flow Cytometry Analysis of Free Intracellular NAD
Acrylamides
/ pharmacology
Biosensing Techniques
/ methods
Calibration
Digitonin
/ pharmacology
Enzyme Inhibitors
/ pharmacology
Flow Cytometry
/ methods
Fluorescence
HeLa Cells
Humans
Hydrogen-Ion Concentration
Intracellular Space
/ metabolism
Mitochondria
/ drug effects
NAD
/ analysis
Nicotinamide Phosphoribosyltransferase
/ antagonists & inhibitors
Piperidines
/ pharmacology
Small Molecule Libraries
/ pharmacology
Statistics as Topic
ARTD
CD-38
NAD+
PARP
circularly permutated fluorescent protein
fluorescent sensor
genetically encoded biosensor
metabolite
nicotinamide adenine dinucleotide
sirtuin
Journal
Current protocols in cytometry
ISSN: 1934-9300
Titre abrégé: Curr Protoc Cytom
Pays: United States
ID NLM: 100899351
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
pubmed:
18
12
2018
medline:
20
7
2019
entrez:
18
12
2018
Statut:
ppublish
Résumé
Flow cytometry approaches combined with a genetically encoded targeted fluorescent biosensor are used to determine the subcellular compartmental availability of the oxidized form of nicotinamide adenine dinucleotide (NAD
Identifiants
pubmed: 30556645
doi: 10.1002/cpcy.54
pmc: PMC6422705
mid: NIHMS998029
doi:
Substances chimiques
Acrylamides
0
Enzyme Inhibitors
0
N-(4-(1-benzoylpiperidin-4-yl)butyl)-3-(pyridin-3-yl)acrylamide
0
Piperidines
0
Small Molecule Libraries
0
NAD
0U46U6E8UK
Nicotinamide Phosphoribosyltransferase
EC 2.4.2.12
Digitonin
KOO5CM684H
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e54Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR000128
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG055431
Pays : United States
Organisme : NIGMS NIH HHS
ID : DP2 GM126897
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS088629
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA069533
Pays : United States
Informations de copyright
© 2018 John Wiley & Sons, Inc.
Références
Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11241-6
pubmed: 10500161
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5203-7
pubmed: 10792038
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3197-202
pubmed: 11248055
Science. 2002 Mar 8;295(5561):1895-7
pubmed: 11847309
Biochem Biophys Res Commun. 2002 Oct 4;297(4):835-40
pubmed: 12359228
Biochem Z. 1956;328(4):252-63
pubmed: 13373833
J Biol Chem. 1963 Apr;238:1475-8
pubmed: 14012814
Structure. 2004 Aug;12(8):1449-59
pubmed: 15296738
J Biol Chem. 2004 Dec 3;279(49):50754-63
pubmed: 15381699
J Biol Chem. 2005 Oct 28;280(43):36334-41
pubmed: 16118205
Biochemistry. 2007 Apr 24;46(16):4912-22
pubmed: 17402747
Cell. 2007 Sep 21;130(6):1095-107
pubmed: 17889652
Nat Methods. 2009 Dec;6(12):875-81
pubmed: 19898485
Cell Mol Life Sci. 2010 Feb;67(3):433-43
pubmed: 19902144
Nat Rev Mol Cell Biol. 2010 Jan;11(1):50-61
pubmed: 19997129
Endocr Rev. 2010 Apr;31(2):194-223
pubmed: 20007326
J Biol Chem. 2010 Oct 29;285(44):34106-14
pubmed: 20724478
J Biol Chem. 2011 Jun 17;286(24):21767-78
pubmed: 21504897
Science. 2011 Sep 30;333(6051):1888-91
pubmed: 21903779
Cell Metab. 2011 Oct 5;14(4):545-54
pubmed: 21982714
Cell Metab. 2011 Oct 5;14(4):555-66
pubmed: 21982715
ACS Chem Biol. 2012 Mar 16;7(3):571-80
pubmed: 22230472
J Neurosci. 2012 Oct 3;32(40):13819-40
pubmed: 23035093
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
PLoS One. 2013 Oct 14;8(10):e76938
pubmed: 24155910
Pharmaceuticals (Basel). 2013 Apr 26;6(5):579-603
pubmed: 24276168
Biochim Biophys Acta. 2014 Mar;1840(3):951-7
pubmed: 24286672
Biochem J. 2014 Nov 15;464(1):13-22
pubmed: 25164254
PLoS One. 2014 Nov 25;9(11):e113939
pubmed: 25423279
Proc Natl Acad Sci U S A. 2015 Mar 3;112(9):2876-81
pubmed: 25730862
Science. 2015 Apr 24;348(6233):453-7
pubmed: 25908823
Cell Metab. 2015 May 5;21(5):777-89
pubmed: 25955212
Science. 2015 Dec 4;350(6265):1208-13
pubmed: 26785480
Science. 2016 Jun 17;352(6292):1474-7
pubmed: 27313049
Biochim Biophys Acta. 2016 Dec;1864(12):1787-1800
pubmed: 27374990
Elife. 2018 May 29;7:
pubmed: 29809136
Methods Mol Biol. 2018;1813:391-414
pubmed: 30097882
Biochem J. 1967 May;103(2):514-27
pubmed: 4291787