Multiple integrin ligands provide a highly adhesive and osteoinductive surface that improves selective cell retention technology.
Adult
Amino Acid Sequence
Animals
Bone Marrow Cells
/ cytology
Bone Matrix
/ metabolism
Cell Adhesion
Cell Differentiation
Cell Proliferation
Cells, Cultured
Collagen
/ chemistry
Humans
Implants, Experimental
Integrins
/ metabolism
Ligands
Male
Mesenchymal Stem Cells
/ cytology
Mice
Osseointegration
Osteogenesis
Peptides
/ chemical synthesis
Surface Properties
Tissue Engineering
/ methods
Tissue Scaffolds
/ chemistry
Young Adult
Bone repair
Cell-adhesive biomaterial
Osteoinductive biomaterial
Selective cell retention
Tissue engineering
Journal
Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
07
09
2018
revised:
11
12
2018
accepted:
13
12
2018
pubmed:
18
12
2018
medline:
20
2
2020
entrez:
18
12
2018
Statut:
ppublish
Résumé
Among various bone tissue engineering strategies, selective cell retention (SCR) technology has been used as a practical clinical method for bone graft manufacturing in real time. The more mesenchymal stem cells (MSCs) are retained, the better the osteoinductive microenvironment provided by the scaffold, which in turn promotes the osteogenesis of the SCR-fabricated bone grafts. Integrin receptors are crucial to cell-matrix adhesion and signal transduction. We designed a collagen-binding domain (CBD)-containing IKVAV-cRGD peptide (CBD-IKVAV-cRGD peptide) to complement the collagen-based demineralized bone matrix (DBM) with a functionalized surface containing multiple integrin ligands, which correspond to the highly expressed integrin subtypes on MSCs. This DBM/CBD-IKVAV-cRGD composite exhibited superior in vitro adhesion capacity to cultured MSCs, as determined by oscillatory cell adhesion assay, centrifugal cell adhesion assay and mimetic SCR. Moreover, it promoted the retention of MSC-like CD271
Identifiants
pubmed: 30557698
pii: S1742-7061(18)30743-8
doi: 10.1016/j.actbio.2018.12.018
pii:
doi:
Substances chimiques
Integrins
0
Ligands
0
Peptides
0
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
106-116Informations de copyright
Copyright © 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.