[Development and evaluation of an in-house database for quick identification of Burkholderia contaminans by MALDI-TOF MS].
Desarrollo y evaluación de una base de datos in house para la identificación rápida de Burkholderia contaminans por EM MALDI-TOF.
Algorithms
Bacterial Proteins
/ analysis
Bacteriological Techniques
Burkholderia
/ chemistry
Burkholderia Infections
/ complications
Cystic Fibrosis
/ complications
Databases, Factual
Humans
Reproducibility of Results
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/ methods
B. contaminans
EM MALDI-TOF
Identificación
Identification
MALDI-TOF MS
Journal
Revista Argentina de microbiologia
ISSN: 0325-7541
Titre abrégé: Rev Argent Microbiol
Pays: Argentina
ID NLM: 8002834
Informations de publication
Date de publication:
Historique:
received:
22
05
2018
revised:
24
09
2018
accepted:
29
09
2018
pubmed:
19
12
2018
medline:
3
3
2020
entrez:
19
12
2018
Statut:
ppublish
Résumé
MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry (MS) proved to be a robust tool for the identification of numerous taxonomic groups. However, it has limitations. A key advantage of this technique is the flexibility for the incorporation of protein profiles of microorganisms not included in the commercial database. Due to the prevalence of Burkholderia contaminans in fibrocystic patients in Argentina and the fact that rapid and reliable microbiological diagnosis is crucial in them, MALDI-TOF MS emerges as a strategic tool. The aim of this work was to develop an additional database with peptide spectra of reference isolates of B. contaminans. This database demonstrated to be successful for the identification of 97% of the isolates analyzed. Therefore, MALDI-TOF MS with the extended database was a useful tool for the identification and differentiation of other related species to B. contaminans.
Identifiants
pubmed: 30558855
pii: S0325-7541(18)30111-1
doi: 10.1016/j.ram.2018.09.001
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Types de publication
Evaluation Study
Journal Article
Langues
spa
Sous-ensembles de citation
IM
Pagination
255-258Informations de copyright
Copyright © 2018 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.