Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity.

Amino Acid Substitution Arabidopsis / enzymology Arabidopsis Proteins / chemistry Coenzyme A Ligases / chemistry Directed Molecular Evolution Mutagenesis
Arabidopsis thaliana LACS Saccharomyces cerevisiae error-prone PCR protein engineering

Journal

The Biochemical journal
ISSN: 1470-8728
Titre abrégé: Biochem J
Pays: England
ID NLM: 2984726R

Informations de publication

Date de publication:
15 01 2019
Historique:
received: 01 10 2018
revised: 11 12 2018
accepted: 17 12 2018
pubmed: 19 12 2018
medline: 5 9 2019
entrez: 19 12 2018
Statut: epublish

Résumé

Long-chain acyl-CoA synthetase (LACS, EC 6.2.1.3) catalyzes the ATP-dependent activation of free fatty acid to form acyl-CoA, which, in turn, serves as the major acyl donor for various lipid metabolic pathways. Increasing the size of acyl-CoA pool by enhancing LACS activity appears to be a useful approach to improve the production and modify the composition of fatty acid-derived compounds, such as triacylglycerol. In the present study, we aimed to improve the enzyme activity of

Identifiants

DOI: 10.1042/BCJ20180787 PMID: 30559328
pubmed: 30559328
pii: BCJ20180787
doi: 10.1042/BCJ20180787
doi:

Substances chimiques

Arabidopsis Proteins 0
Coenzyme A Ligases EC 6.2.1.-
LACS9 protein, Arabidopsis EC 6.2.1.3

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

151-164

Informations de copyright

© 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

Auteurs

Yang Xu (Y)

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.

Kristian Mark P Caldo (KMP)

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.

Roman Holic (R)

Centre of Biosciences, Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Dúbravská Cesta 9, 840 05 Bratislava, Ivanka pri Dunaji 900 28, Slovakia.

Elzbieta Mietkiewska (E)

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.

Jocelyn Ozga (J)

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.

Syed Masood Rizvi (SM)

Corteva Agriscience, Agriculture Division of DowDuPont, Site 600, RR #6 PO Box 12, Saskatoon, Saskatchewan, Canada S7K 3J9.

Guanqun Chen (G)

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
ORCID: 0000-0001-5790-3903

Randall J Weselake (RJ)

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5 randall.weselake@ualberta.ca.
ORCID: 0000-0001-8699-690X

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Substitution d'acide aminé › Engineering Arabidopsis long-chain acyl-CoA...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Engineering Arabidopsis long-chain acyl-CoA...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Engineering Arabidopsis long-chain acyl-CoA...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Carbon-sulfur ligases › Coenzyme A ligases › Engineering Arabidopsis long-chain acyl-CoA...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Évolution moléculaire dirigée › Engineering Arabidopsis long-chain acyl-CoA...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Engineering Arabidopsis long-chain acyl-CoA...