Asymmetric division through a reduction of microtubule centering forces.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
04 03 2019
04 03 2019
Historique:
received:
13
07
2018
revised:
30
10
2018
accepted:
30
11
2018
pubmed:
20
12
2018
medline:
27
2
2020
entrez:
20
12
2018
Statut:
ppublish
Résumé
Asymmetric divisions are essential for the generation of cell fate and size diversity. They implicate cortical domains where minus end-directed motors, such as dynein, are activated to pull on microtubules to decenter asters attached to centrosomes, nuclei, or spindles. In asymmetrically dividing cells, aster decentration typically follows a centering phase, suggesting a time-dependent regulation in the competition between microtubule centering and decentering forces. Using symmetrically dividing sea urchin zygotes, we generated cortical domains of magnetic particles that spontaneously cluster endogenous dynein activity. These domains efficiently attract asters and nuclei, yielding marked asymmetric divisions. Remarkably, aster decentration only occurred after asters had first reached the cell center. Using intracellular force measurement and models, we demonstrate that this time-regulated imbalance results from a global reduction of centering forces rather than a local maturation of dynein activity at the domain. Those findings define a novel paradigm for the regulation of division asymmetry.
Identifiants
pubmed: 30563876
pii: jcb.201807102
doi: 10.1083/jcb.201807102
pmc: PMC6400563
doi:
Substances chimiques
Dyneins
EC 3.6.4.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
771-782Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019 Sallé et al.
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